BMRB Entry 50817

Title:
1H, 13C and 15N Backbone Chemical Shift Assignments of the Y526A SH3 variant of the JNK-interacting protein 1
Deposition date:
2021-03-10
Original release date:
2021-12-13
Authors:
Marino Perez, Laura; Ringkjobing Jensen, Malene
Citation:

Citation: Marino Perez, Laura; Ielasi, Francesco; Mamigonian Bessa, Luiza; Maurin, Damien; Kragelj, Jaka; Blackledge, Martin; Salvi, Nicola; Bouvignies, Guillaume; Palencia, Andres; Jensen, Malene. "Visualizing protein breathing motions associated with aromatic ring flipping"  Nature 602, 695-700 (2022).
PubMed: 35173330

Assembly members:

Assembly members:
entity_1, polymer, 63 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET28a

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts177
15N chemical shifts57
1H chemical shifts57

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1JIP1-SH3-Y526A1

Entities:

Entity 1, JIP1-SH3-Y526A 63 residues - Formula weight is not available

1   GLYHISMETGLUGLNTHRHISARGALAILE
2   PHEARGPHEVALPROARGHISGLUASPGLU
3   LEUGLULEUGLUVALASPASPPROLEULEU
4   VALGLULEUGLNALAGLUASPTYRTRPALA
5   GLUALATYRASNMETARGTHRGLYALAARG
6   GLYVALPHEPROALATYRTYRALAILEGLU
7   VALTHRLYS

Samples:

sample_1: JIP1-SH3-Y526A, [U-95% 13C; U-95% 15N], 1.06 mM; NaCl 150 mM; HEPES 50 mM

sample_conditions_1: pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D HNCOsample_1isotropicsample_conditions_1
3D HN(CO)CACBsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1

Software:

NMRPipe - processing

NMRFAM-SPARKY - chemical shift assignment, data analysis, peak picking

TOPSPIN - collection

NMR spectrometers:

  • Bruker AVANCE III 700 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks