BMRB Entry 19666

Title:
Structure of insect-specific sodium channel toxin mu-Dc1a
Deposition date:
2013-12-08
Original release date:
2014-07-14
Authors:
Bende, Niraj; Mobli, Mehdi; King, Glenn
Citation:

Citation: Bende, Niraj; Dziemborowicz, Slawomir; Mobli, Mehdi; Herzig, Volker; Gilchrist, John; Nicholson, Graham; King, Glenn; Bosmans, Frank. "A distinct voltage-sensor locus determines insect-family selectivity of a unique sodium channel modulator found in desert bush spider venom"  Nat. Commun. 5, 1-10 (2014).
PubMed: 25014760

Assembly members:

Assembly members:
Dc1a, polymer, 57 residues, 6502.466 Da.

Natural source:

Natural source:   Common Name: desert bush spider   Taxonomy ID: 38407   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Diguetia canities

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pLIC

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts245
15N chemical shifts60
1H chemical shifts374

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Dc1a1

Entities:

Entity 1, Dc1a 57 residues - 6502.466 Da.

1   SERALALYSASPGLYASPVALGLUGLYPRO
2   ALAGLYCYSLYSLYSTYRASPVALGLUCYS
3   ASPSERGLYGLUCYSCYSGLNLYSGLNTYR
4   LEUTRPTYRLYSTRPARGPROLEUASPCYS
5   ARGCYSLEULYSSERGLYPHEPHESERSER
6   LYSCYSVALCYSARGASPVAL

Samples:

sample_1: Dc1a, [U-99% 13C; U-99% 15N], 350 uM; sodium acetate 20 mM; H2O 95%; D2O 5%

sample_conditions_1: ionic strength: 0.02 M; pH: 5.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D 1H-13C NOESY aliphaticsample_1isotropicsample_conditions_1
3D 1H-13C NOESY aromaticsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
4D HCC(CO)NHsample_1isotropicsample_conditions_1

Software:

CYANA v3, Guntert, Mumenthaler and Wuthrich - structure solution

TALOS v+, Cornilescu, Delaglio and Bax - geometry optimization

XEASY, Bartels et al. - chemical shift assignment

TOPSPIN v3, Bruker Biospin - collection

Rowland_NMR_Toolkit v3, Jeffrey C. Hoch, Alan S. Stern - processing

GENPROC v2, Mehdi Mobli - processing

NMR spectrometers:

  • Bruker Avance 900 MHz

Related Database Links:

UNP P49126
PDB
GB AAB60253
SP P49126
AlphaFold P49126 P49126

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks