BMRB Entry 19114

Name: 4E-BP2
Published: 2014-02-12

Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR19114

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

Select model:

I am color blind

Download raw data. Direct link to this display. Help

Title:

4E-BP2

Deposition date:

2013-03-27

Original release date:

2014-02-12

Authors:

Forman-Kay, Julie; Bah, Alaji

Citation:

Citation: Lukhele, Sabelo; Bah, Alaji; Lin, Hong; Sonenberg, Nahum; Forman-Kay, Julie. "Interaction of the eukaryotic initiation factor 4E with 4E-BP2 at a dynamic bipartite interface." Structure 21, 2186-2196 (2013).
PubMed: 24207126

Assembly members:

Assembly members:
4E-BP2, polymer, 128 residues, 13798.3 Da.

Natural source:

Natural source: Common Name: Humans Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pGEX

Entity Sequences (FASTA):

Entity Sequences (FASTA):
4E-BP2: MPLGSPEFMSSSAGSGHQPS QSRAIPTRTVAISDAAQLPH DYCTTPGGTLFSTTPGGTRI IYDRKFLLDRRNSPMAQTPP CHLPNIPGVTSPGTLIEDSK VEVNNLDNLDNHDRKHAVGD DAQFEMDI

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	438
15N chemical shifts	113
1H chemical shifts	209

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	4E-BP2, native	1
2	4E-BP2, intrinsically disordered	1

Entities:

Entity 1, 4E-BP2, native 128 residues - 13798.3 Da.

Residues -6 to 0 is a tag remaining after cleaving of GST fusion protein.

1

MET

PRO

LEU

GLY

SER

PRO

GLU

PHE

MET

SER

2

SER

ALA

GLY

SER

GLY

HIS

GLN

PRO

SER

3

GLN

SER

ARG

ALA

ILE

PRO

THR

ARG

THR

VAL

4

ALA

ILE

SER

ASP

ALA

GLN

LEU

PRO

HIS

5

ASP

TYR

CYS

THR

PRO

GLY

THR

LEU

6

PHE

SER

THR

PRO

GLY

THR

ARG

ILE

7

ILE

TYR

ASP

ARG

LYS

PHE

LEU

ASP

ARG

8

ARG

ASN

SER

PRO

MET

ALA

GLN

THR

PRO

9

CYS

HIS

LEU

PRO

ASN

ILE

PRO

GLY

VAL

THR

10

SER

PRO

GLY

THR

LEU

ILE

GLU

ASP

SER

LYS

11

VAL

GLU

VAL

ASN

LEU

ASP

ASN

LEU

ASP

12

ASN

HIS

ASP

ARG

LYS

HIS

ALA

VAL

GLY

ASP

13

ASP

ALA

GLN

PHE

GLU

MET

ASP

ILE

Samples:

sample_1: 4E-BP2, [U-99% 13C; U-99% 15N], 800 – 1200 uM; DTT 2 mM; sodium chloride 100 mM; sodium phosphate 30 mM; EDTA 1 mM; benzamidine 1 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 150 mM; pH: 6.0; pressure: 1 atm; temperature: 283 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1

Software:

SPARKY, Goddard - chemical shift assignment

NMR spectrometers:

Varian INOVA 800 MHz

BMRB	19905
DBJ	BAG11409 BAG36466
EMBL	CAG47036
GB	AAA62270 AAH05057 AAH50633 AAP35981 AAX32712
PRF	2021415B
REF	NP_001178078 NP_001244839 NP_004087 XP_002718482 XP_003133106
SP	Q13542
TPG	DAA14291
AlphaFold	Q13542