BMRB Entry 7365

Title:
Solution structure of Fe65 WW domain
Deposition date:
2007-01-19
Original release date:
2009-10-15
Authors:
Kouno, T.; Mizuguchi, M.; Nozawa, Y.; Sugaya, T.; Kawano, K.
Citation:

Citation: Kouno, T.; Mizuguchi, M.; Nozawa, Y.; Sugaya, T.; Kawano, K.. "Solution structure of Fe65 WW domain"  to be published ., .-..

Assembly members:

Assembly members:
Amyloid_beta_A4_precursor_protein-binding_family_B_member_1, polymer, 55 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Amyloid_beta_A4_precursor_protein-binding_family_B_member_1: GPLGSDSFWNPNAFETDSDL PAGWMRVQDTSGTYYWHIPT GTTQWEPPGRASPSQ

Data sets:
Data typeCount
13C chemical shifts131
15N chemical shifts50
1H chemical shifts313

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1WW domain1

Entities:

Entity 1, WW domain 55 residues - Formula weight is not available

1   GLYPROLEUGLYSERASPSERPHETRPASN
2   PROASNALAPHEGLUTHRASPSERASPLEU
3   PROALAGLYTRPMETARGVALGLNASPTHR
4   SERGLYTHRTYRTYRTRPHISILEPROTHR
5   GLYTHRTHRGLNTRPGLUPROPROGLYARG
6   ALASERPROSERGLN

Samples:

sample_1: WW domain, [U-13C; U-15N], 0.65 mM; phosphate buffer 10 mM; D2O 10%; H2O 90%

sample_2: WW domain, [U-13C; U-15N], 0.65 mM; phosphate buffer 10 mM; D2O 100%

sample_conditions_1: pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D_15N-separated_NOESYsample_1isotropicsample_conditions_1
HNHAsample_1isotropicsample_conditions_1
3D_13C-separated_NOESYsample_2isotropicsample_conditions_1
2D NOESYsample_2isotropicsample_conditions_1

Software:

NMRPipe v2.2, F. Delaglio, et al. - processing

PIPP v4.3.2, D.S. Garret - collection

X-PLOR v3.1f, A.T. Brunger - structure solution

X-PLOR v3.1f, A.T. Brunger - refinement

NMR spectrometers:

  • Bruker AVANCE 800 MHz

Related Database Links:

PDB
DBJ BAH11531 BAH11532 BAH11554 BAH11576 BAH11788
EMBL CAA42998 CAD98057
GB AAK18816 ABL07489 EPY80480 KFO24725
REF NP_001240816 NP_001244248 NP_001244249 NP_001244250 NP_001244252

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks