BMRB Entry 7362

Title:
NMR Structure of Protein UPF0165 protein AF_2212 from Archaeoglobus Fulgidus; Northeast Structural Genomics Consortium Target GR83
Deposition date:
2007-01-19
Original release date:
2010-03-04
Authors:
Singarapu, K.; Sukumaran, D.; Parish, D.; Atreya, H.; Liu, G.; Eletsky, A.; Chen, C.; Jiang, M.; Cunningham, K.; Xiao, R.; Swapna, G.; Acton, T.; Rost, B.; Montelione, G.; Szyperski, T.
Citation:

Citation: Singarapu, K.; Sukumaran, D.; Parish, D.; Atreya, H.; Liu, G.; Eletsky, A.; Chen, C.; Jiang, M.; Cunningham, K.; Ma, L.; Xiao, R.; Liu, J.; Baran, M.; Swapna, G.; Acton, T.; Rost, B.; Montelione, G.; Szyperski, T.. "NMR Structure of Protein Y2212_ARCFU from Archaeoglobus Fulgidus; Northeast Structural Genomics Consortium Target GR83"  .

Assembly members:

Assembly members:
UPF0165_protein_AF_2212, polymer, 69 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Archaeoglobus fulgidus   Taxonomy ID: 2234   Superkingdom: Archaea   Kingdom: not available   Genus/species: Archaeoglobus fulgidus

Experimental source:

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
UPF0165_protein_AF_2212: MPKIIEAVYENGVFKPLQKV DLKEGERVKIKLELKVEPID LGEPVSVEEIKKIRDGTWMS SLEHHHHHH

Data sets:
Data typeCount
13C chemical shifts229
15N chemical shifts62
1H chemical shifts477

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1UPF0165 chain A1
2UPF0165 chain B1

Entities:

Entity 1, UPF0165 chain A 69 residues - Formula weight is not available

1   METPROLYSILEILEGLUALAVALTYRGLU
2   ASNGLYVALPHELYSPROLEUGLNLYSVAL
3   ASPLEULYSGLUGLYGLUARGVALLYSILE
4   LYSLEUGLULEULYSVALGLUPROILEASP
5   LEUGLYGLUPROVALSERVALGLUGLUILE
6   LYSLYSILEARGASPGLYTHRTRPMETSER
7   SERLEUGLUHISHISHISHISHISHIS

Samples:

sample: UPF0165, [U-13C; U-15N], 1.2 mM; NaCl 5 mM; NH4OAc 20 mM; DTT 10 mM; NaN3 0.02%; D2O 5%; H2O 95%

sample_conditions_1: ionic strength: 100 mM; pH: 5.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
4,3D, GFT HNNCABCAsampleisotropicsample_conditions_1
4,3D, GFT CABCACoNNHsampleisotropicsample_conditions_1
4,3D, GFT HCCH COSYsampleisotropicsample_conditions_1
3D, 15N-13C resolved simultanious NOESYsampleisotropicsample_conditions_1
3D, 15N, 13C resolved X-filtered NOESYsampleisotropicsample_conditions_1

Software:

NMRPipe v2.3, Delaglio et al - processing

DYANA v1.5, Guntert et al - data analysis

CYANA v2.1, Guntert et al - refinement

CNS v1.1, Brunget et al - refinement

NMR spectrometers:

  • Varian INOVA 750 MHz
  • Varian INOVA 600 MHz

Related Database Links:

PDB
GB AAB89054 AIG99201
REF NP_071037 WP_010879701
SP O28071
AlphaFold O28071

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks