BMRB Entry 7296

Title:
Solution Conformation of the His 47 to Ala 47 Mutant of Pseudomonas stutzeri ZoBell Ferrocytochrome c-551
Deposition date:
2006-09-14
Original release date:
2007-10-09
Authors:
Liang, Qiaoli; Miller, Gregory; Beeghley, Chanda; Graf, Coyner; Timkovich, Russell
Citation:

Citation: Liang, Qiaoli; Miller, Gregory; Beeghley, Chanda; Graf, Coyner; Timkovich, Russell. "Solution conformation of the His-47 to Ala-47 mutant of Pseudomonas stutzeri ZoBell ferrocytochrome c-551."  Biophys. J. 93, 1700-1706 (2007).
PubMed: 17496029

Assembly members:

Assembly members:
H47A peptide chain, polymer, 82 residues, 9500 Da.
HEM, non-polymer, 616.487 Da.

Natural source:

Natural source:   Common Name: Pseudomonas stutzeri Zobell   Taxonomy ID: 316   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Pseudomonas stutzeri

Experimental source:

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):

Data sets:
Data typeCount
1H chemical shifts596

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1H47A cyt c-5511
2PROTOPORPHYRIN_IX_CONTAINING_FE2

Entities:

Entity 1, H47A cyt c-551 82 residues - 9500 Da.

1   GLNASPGLYGLUALALEUPHELYSSERLYS
2   PROCYSALAALACYSHISSERVALASPTHR
3   LYSMETVALGLYPROALALEULYSGLUVAL
4   ALAALALYSASNALAGLYVALGLUGLYALA
5   ALAASPTHRLEUALALEUALAILELYSASN
6   GLYSERGLNGLYVALTRPGLYPROILEPRO
7   METPROPROASNPROVALTHRGLUGLUGLU
8   ALALYSILELEUALAGLUTRPVALLEUSER
9   LEULYS

Entity 2, PROTOPORPHYRIN_IX_CONTAINING_FE - C34 H32 Fe N4 O4 - 616.487 Da.

1   HEM

Samples:

sample_1: H47A c-551 1.5 mM; H2O 10%; D2O 90%; sodium phosphate buffer 50 mM; sodium dithionite 10 mM

conditions_1: pH: 7.00; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
NOESYsample_1not availableconditions_1
DQF-COSYsample_1not availableconditions_1
TOCSYsample_1not availableconditions_1

Software:

NMRView v6.2.10 -

NMRPipe vJuly, 2004 -

ARIA v2.0alpha -

CNS v1.1 -

NMR spectrometers:

  • Bruker AVANCE 600 MHz