BMRB Entry 7262

Title:
NMR assignments of the the N-terminal subdomain of IGFBP-6
Deposition date:
2006-08-10
Original release date:
2007-03-06
Authors:
Chandrashekaran, Indu; Yao, Shenggen; Wang, Chunxiao; Bansal, Paramjit; Alewood, Paul; Wallace, John; Bach, Leon; Norton, Raymond
Citation:

Citation: Chandrashekaran, Indu; Yao, Shenggen; Wang, Chunxiao; Bansal, Paramjit; Alewood, Paul; Forbes, B.; Wallace, John; Bach, Leon; Norton, Raymond. "The N-Terminal Subdomain of Insulin-like Growth Factor (IGF) Binding Protein 6. Structure and Interaction with IGFs"  Biochemistry 46, 3065-3074 (2007).
PubMed: 17305365

Assembly members:

Assembly members:
NN-BP-6, polymer, 45 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
NN-BP-6: ALARCPGCGQGVQAGCPGGC VEEEDGGSPAEGCAEAEGCL RREGQ

Data sets:
Data typeCount
13C chemical shifts37
15N chemical shifts35
1H chemical shifts199
coupling constants15

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
11-45IGFBP-61

Entities:

Entity 1, 1-45IGFBP-6 45 residues - Formula weight is not available

1   ALALEUALAARGCYSPROGLYCYSGLYGLN
2   GLYVALGLNALAGLYCYSPROGLYGLYCYS
3   VALGLUGLUGLUASPGLYGLYSERPROALA
4   GLUGLYCYSALAGLUALAGLUGLYCYSLEU
5   ARGARGGLUGLYGLN

Samples:

sample_1: NN-BP-6 1.1 mM; Sodium acetate 10 mM; Sodium azide 0.02%; D2O 5%

conditions_1: ionic strength: 10 mM; pH: 4.3; temperature: 288 K

Experiments:

NameSampleSample stateSample conditions
1H-13C HMQCsample_1not availableconditions_1
2D 1H-1H NOESYsample_1not availableconditions_1
2D 1H-1H DQF-COSYsample_1not availableconditions_1
2D 1H-1H TOCSYsample_1not availableconditions_1
1H15N HSQCsample_1not availableconditions_1

Software:

TOPSPIN v1.3, Bruker -

XEASY v1.3 -

NMR spectrometers:

  • Bruker Avance 500 MHz
  • Bruker DRX 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks