BMRB Entry 7257

Title:
Solution structure of the RRM domain of SR rich factor 9G8
Deposition date:
2006-08-10
Original release date:
2006-12-20
Authors:
Tintaru, A.; Hautbergue, G.; Golovanov, A.; Lian, L.; Wilson, S.
Citation:

Citation: Hargous, Y.; Hautbergue, G.; Tintaru, A.; Skrisovska, L.; Golovanov, A.; Stevenin, J.; Lian, L.; Wilson, S.; Allain, F.. "Molecular basis of RNA recognition and TAP binding by the SR proteins SRp20 and 9G8"  EMBO J. 25, 5126-5137 (2006).
PubMed: 17036044

Assembly members:

Assembly members:
RRM domain of SR rich factor 9G8, polymer, 101 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts444
15N chemical shifts109
1H chemical shifts697

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1RRM domain of SR rich factor 9G81

Entities:

Entity 1, RRM domain of SR rich factor 9G8 101 residues - Formula weight is not available

1   METLYSVALTYRVALGLYASNLEUGLYTHR
2   GLYALAGLYLYSGLYGLULEUGLUARGALA
3   PHESERTYRTYRGLYPROLEUARGTHRVAL
4   TRPILEALAARGASNPROPROGLYPHEALA
5   PHEVALGLUPHEGLUASPPROARGASPALA
6   GLUASPALAVALARGGLYLEUASPGLYLYS
7   VALILECYSGLYSERARGVALARGVALGLU
8   LEUSERTHRGLYMETPROARGARGSERARG
9   PHEASPARGPROPROALAARGARGLYSLEU
10   LEUGLUVALLEUPHEASNGLYPROLEUGLU
11   HIS

Samples:

sample_1: RRM domain of SR rich factor 9G8, [U-13C; U-15N], 0.6 mM; CH3COONa 20 mM; L-ARG 25 mM; L-Glu 50 mM; EDTA 5 mM; DTT 10 mM; H2O 90%; D2O 10%

sample_cond_1: pH: 5.5; pressure: 1 atm; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
3D 13C-separated NOESYnot availablenot availablenot available
3D 15N-separated NOESYnot availablenot availablenot available
2D TOCSYnot availablenot availablenot available

Software:

xwinnmr v3.5 - collection

NMRPipe v1.0 - processing

NMRView v5.01 - data analysis

CYANA v2.1 - structure solution

AMBER v7.0 - refinement

NMR spectrometers:

  • Bruker DRX 600 MHz

Related Database Links:

PDB
DBJ BAC03661 BAC28058 BAC32521 BAC38650 BAE01828
GB AAA35495 AAA88098 AAH00997 AAH14857 AAH17369
REF NP_001026854 NP_001029449 NP_001034124 NP_001086749 NP_001182375
SP Q16629 Q3T106 Q8BL97
TPG DAA24684
AlphaFold Q8BL97 Q16629 Q3T106

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks