BMRB Entry 7185

Title:
An ARC/MEDIATOR subunit required for SREBP gene activation and regulation of cholesterol and fatty acid homeostasis
Deposition date:
2006-06-21
Original release date:
2006-10-11
Authors:
Wagner, G.; Vought, B.; Sun, Y.J.; Hyberts, S.
Citation:

Citation: Yang, F.; Vought, B.; Satterlee, J.; Walker, A.; Jim_Sun, Z.; Watts, J.; DeBeaumont, R.; Saito, R.; Hyberts, S.; Yang, S.; Macol, C.; Iyer, L.; Tjian, R.; van den Heuvel, S.; Hart, A.; Wagner, G.; Naar, A.. "An ARC/Mediator subunit required for SREBP control of cholesterol and lipid homeostasis"  Nature 442, 700-704 (2006).
PubMed: 16799563

Assembly members:

Assembly members:
Positive cofactor 2 glutamine/Q-rich-associated protein, polymer, 77 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Positive cofactor 2 glutamine/Q-rich-associated protein: GAMGQETDWRSTAFRQKLVS QIEDAMRKAGVAHSKSSKDM ESHVFLKAKTRDEYLSLVAR LIIHFRDIHNKKSQASV

Data sets:
Data typeCount
13C chemical shifts254
15N chemical shifts75
1H chemical shifts388

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Positive cofactor 2 glutamine/Q-rich-associated protein1

Entities:

Entity 1, Positive cofactor 2 glutamine/Q-rich-associated protein 77 residues - Formula weight is not available

1   GLYALAMETGLYGLNGLUTHRASPTRPARG
2   SERTHRALAPHEARGGLNLYSLEUVALSER
3   GLNILEGLUASPALAMETARGLYSALAGLY
4   VALALAHISSERLYSSERSERLYSASPMET
5   GLUSERHISVALPHELEULYSALALYSTHR
6   ARGASPGLUTYRLEUSERLEUVALALAARG
7   LEUILEILEHISPHEARGASPILEHISASN
8   LYSLYSSERGLNALASERVAL

Samples:

sample_1: Positive cofactor 2 glutamine/Q-rich-associated protein, [U-15N; U-13C], 1 mM; NaCl 30 mM; phosphate buffer 10 mM; H2O 90%; D2O 10%

sample_cond_1: ionic strength: 30 mM; pH: 6.8; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
HNCAsample_1not availablesample_cond_1
HNCOCAsample_1not availablesample_cond_1
HNCACBsample_1not availablesample_cond_1
HNCOCACBsample_1not availablesample_cond_1
HNCOsample_1not availablesample_cond_1
HNCACOsample_1not availablesample_cond_1
HN(COCA)NHsample_1not availablesample_cond_1
N15noesysample_1not availablesample_cond_1
tocsysample_1not availablesample_cond_1
CCONHsample_1not availablesample_cond_1
HCCONHsample_1not availablesample_cond_1
aliphaticsample_1not availablesample_cond_1
aromatic-C13noesysample_1not availablesample_cond_1
HCCHTOCSYsample_1not availablesample_cond_1

Software:

PROSA v3.7 - processing

CARA v1.5 - data analysis

CYANA v2.1 - refinement, structure solution

NMR spectrometers:

  • Bruker AVANCE 500 MHz

Related Database Links:

PDB
DBJ BAC03446 BAD21436 BAE38826 BAE41448 BAG57747
EMBL CAG30423 CAK54568 CAK54867
GB AAC12944 AAH03078 AAH13985 AAH54779 AAI42513
REF NP_001003891 NP_001035773 NP_001092645 NP_001101795 NP_001267180
SP Q924H2 Q96RN5
TPG DAA20413
AlphaFold Q96RN5 Q924H2