BMRB Entry 7120

Title:
The SAM domain of DLC1 defines a novel structure that interacts with elongation factor 1A1 at cortical actin and membrane ruffles
Deposition date:
2006-05-18
Original release date:
2009-05-27
Authors:
Yang, S.
Citation:

Citation: Zhong, D.; Yang, S.; Zhang, J.; Buschdorf, J.; Yang, D.; Low, B.. "The SAM domain of DLC1 defines a novel structure that interacts with elongation factor 1A1 at cortical actin and membrane ruffles"  .

Assembly members:

Assembly members:
Rho-GTPase-activating protein 7, polymer, 76 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Rho-GTPase-activating protein 7: MCRKKPDTMILTQIEAKEAC DWLRATGFPQYAQLYEDFLF PIDISLVKREHDFLDRDAIE ALCRRLNTLNKCAVMK

Data sets:
Data typeCount
13C chemical shifts256
15N chemical shifts76
1H chemical shifts511

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Deleted in liver cancer 1 protein, isoform 21

Entities:

Entity 1, Deleted in liver cancer 1 protein, isoform 2 76 residues - Formula weight is not available

1   METCYSARGLYSLYSPROASPTHRMETILE
2   LEUTHRGLNILEGLUALALYSGLUALACYS
3   ASPTRPLEUARGALATHRGLYPHEPROGLN
4   TYRALAGLNLEUTYRGLUASPPHELEUPHE
5   PROILEASPILESERLEUVALLYSARGGLU
6   HISASPPHELEUASPARGASPALAILEGLU
7   ALALEUCYSARGARGLEUASNTHRLEUASN
8   LYSCYSALAVALMETLYS

Samples:

sample_1: Rho-GTPase-activating protein 7, [U-15N; U-13C], 1 mM; sodium phosphate buffer 50 mM; DTT 3 mM; H2O 90%; D2O 10%

sample_2: Rho-GTPase-activating protein 7, [U-15N], 1 mM; sodium phosphate buffer 50 mM; DTT 3 mM; H2O 90%; D2O 10%

sample_cond_1: ionic strength: 50 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D 13C-separated NOESYnot availablenot availablesample_cond_1
3D 15N-separated NOESYnot availablenot availablesample_cond_1

Software:

xwinnmr v3.5 - collection

NMRView v5.2.2_01 - data analysis

NMRPipe v2004.126.16.02 - processing

AMBER v7 - refinement

TALOS v2003.027.13.05 - refinement

CYANA v1.0.5 - structure solution

NMR spectrometers:

  • Bruker AVANCE 500 MHz

Related Database Links:

PDB
DBJ BAB21814 BAF82423 BAF84371 BAG10131
GB AAB81637 AAB87700 AAH54511 AAK97501 ACE86794
PIR G59435
REF NP_006085 NP_872584 XP_001092830 XP_002919590 XP_003256738
SP Q96QB1
AlphaFold Q96QB1

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks