BMRB Entry 7075

Title:
Assignment of conserved hypothetical protein pa2412
Deposition date:
2006-04-17
Original release date:
2006-10-20
Authors:
Lemak, Alexander; Yee, Adelinda; Lukin, Jonathan; Semesi, Anthony; Arrowsmith, Cheryl
Citation:

Citation: Lemak, Alexander; Lukin, Jonathan; Yee, Adelinda; Semesi, Anthony; Arrowsmith, Cheryl. "Solution structure of pa2412 protein determined using ABACUS protocol"  The BMRB entry is the only known published source for the data..

Assembly members:

Assembly members:
conserved hypothetical protein pa2412, polymer, 72 residues, 8443 Da.

Natural source:

Natural source:   Common Name: Pseudomonas aeruginosa   Taxonomy ID: 15597608   Superkingdom: Bacteria   Kingdom: not available   Genus/species: pseudomonas aeruginosa

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: P11

Entity Sequences (FASTA):

Entity Sequences (FASTA):
conserved hypothetical protein pa2412: MTSVFDRDDIQFQVVVNHEE QYSIWPEYKEIPQGWRAAGK SGLKKDCLAYIEEVWTDMRP LSLRQHMDKAAG

Data sets:
Data typeCount
13C chemical shifts285
15N chemical shifts70
1H chemical shifts459

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1pa24121

Entities:

Entity 1, pa2412 72 residues - 8443 Da.

1   METTHRSERVALPHEASPARGASPASPILE
2   GLNPHEGLNVALVALVALASNHISGLUGLU
3   GLNTYRSERILETRPPROGLUTYRLYSGLU
4   ILEPROGLNGLYTRPARGALAALAGLYLYS
5   SERGLYLEULYSLYSASPCYSLEUALATYR
6   ILEGLUGLUVALTRPTHRASPMETARGPRO
7   LEUSERLEUARGGLNHISMETASPLYSALA
8   ALAGLY

Samples:

sample_1: conserved hypothetical protein pa2412, [U-13C; U-15N], 0.6 mM; NaCl 450 mM; MOPS 10 mM

cond_set1: ionic strength: 0.45 M; pH: 6.5; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
15N HSQCnot availablenot availablenot available
HNCOnot availablenot availablenot available
CBCAcoNHnot availablenot availablenot available
HBHAcoNHnot availablenot availablenot available
CCcoNH TOCSYnot availablenot availablenot available
HCCcoNH TOCSYnot availablenot availablenot available
13C HSQCnot availablenot availablenot available
HC(C)H TOCSYnot availablenot availablenot available
HCC(H) TOCSYnot availablenot availablenot available
15N NOESYnot availablenot availablenot available
13C NOESYnot availablenot availablenot available

Software:

SPARKY v3.106 - assignemt, spectral analysis

NMR spectrometers:

  • Bruker AVANCE 600 MHz
  • Bruker AVANCE 500 MHz

Related Database Links:

PDB
DBJ BAK90265 BAP23198 BAP50810 GAA18000 GAJ51291
EMBL CAW27611 CCQ87246 CDH74417 CDH77199 CDI90691
GB AAG05800 AAT50634 ABJ11588 ABR86930 AEO75113
REF NP_251102 WP_003089636 WP_023084226 WP_033996385 WP_034025318

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks