BMRB Entry 7061

Title:
NMR structure of talin-PTB in complex with PIPKI
Deposition date:
2006-04-10
Original release date:
2008-07-17
Authors:
Kong, X.; Wang, X.; Misra, S.; Qin, J.
Citation:

Citation: Kong, X.; Wang, X.; Misra, S.; Qin, J.. "Structural Basis for the Phosphorylation-regulated Focal Adhesion Targeting of Type Igamma Phosphatidylinositol Phosphate Kinase (PIPKIgamma) by Talin"  J. Mol. Biol. 359, 47-54 (2006).
PubMed: 16616931

Assembly members:

Assembly members:
Talin-1, polymer, 106 residues, Formula weight is not available
SWVXSPLH, polymer, 8 residues, 261.168 Da.

Natural source:

Natural source:   Common Name: Mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculus

Experimental source:

Experimental source:   Production method: recombinant technology

Data sets:
Data typeCount
13C chemical shifts441
15N chemical shifts123
1H chemical shifts740

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Talin-11
2peptide2

Entities:

Entity 1, Talin-1 106 residues - Formula weight is not available

1   LEULYSTHRTYRGLYVALSERPHEPHELEU
2   VALLYSGLULYSMETLYSGLYLYSASNLYS
3   LEUVALPROARGLEULEUGLYILETHRLYS
4   GLUCYSVALMETARGVALASPGLULYSTHR
5   LYSGLUVALILEGLNGLUTRPSERLEUTHR
6   ASNILELYSARGTRPALAALASERPROLYS
7   SERPHETHRLEUASPPHEGLYASPTYRGLN
8   ASPGLYTYRTYRSERVALGLNTHRTHRGLU
9   GLYGLUGLNILEALAGLNLEUILEALAGLY
10   TYRILEASPILEILELEULYSLYSLYSLYS
11   SERLYSASPHISPHEGLY

Entity 2, peptide 8 residues - 261.168 Da.

1   SERTRPVALPTRSERPROLEUHIS

Samples:

sample_1: Talin-1, [U-13C; U-15N], 1 mM; SWVXSPLH 1 mM; phosphate buffer 20 mM; Ca2+ 5 mM; D2O 10%; H2O 90%

sample_cond_1: pH: 6.3; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
HNCACBsample_1not availablesample_cond_1
CBCA(CO)NHsample_1not availablesample_cond_1
HCCH-TOCSYsample_1not availablesample_cond_1
HNCOsample_1not availablesample_cond_1
edited NOESYsample_1not availablesample_cond_1
filtered NOESYsample_1not availablesample_cond_1

Software:

TALOS - data analysis

X-PLOR - refinement

NMR spectrometers:

  • VARIAN INOVA 600 MHz

Related Database Links:

BMRB 15792
PDB
DBJ BAA82979 BAC30516 BAC65702 BAE27781 BAG09941
EMBL CAA39588 CAI10978 CAM17190
GB AAD13152 AAF23322 AAF27330 AAH42923 AAI21760
PRF 1617167A
REF NP_001034114 NP_006280 NP_035732 NP_989854 XP_001084941
SP P26039 P54939 Q9Y490
AlphaFold P54939 P26039 Q9Y490

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks