BMRB Entry 7059

Title:
1H, 13C, and 15N Resonance Assignments of the Pyrazinamidase from Mycobacterium Tuberculosis
Deposition date:
2006-04-10
Original release date:
2006-10-02
Authors:
Son, Woo-Sung; Kim, Won-Je; Kim, Yong-Jin; Suh, Se-Won; Lee, Bong-Jin
Citation:

Citation: Son, Woo-Sung; Kim, Won-Je; Kim, Yong-Jin; Suh, Se-Won; Ikegami, Takahisa; Akutsu, Hideo; Lee, Bong-Jin. "1H, 13C and 15N Resonance Assignments of the Pyrazinamidase from Mycobacterium Tuberculosis"  J. Biomol. NMR 36, 60-60 (2006).
PubMed: 17131033

Assembly members:

Assembly members:
Pyrazinamidase, polymer, 186 residues, 19604.6 Da.

Natural source:

Natural source:   Common Name: Mycobacterium Tuberculosis   Taxonomy ID: 1773   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Mycobacterium tuberculosis

Experimental source:

Experimental source:   Production method: recombinant technology   Vector: pET-22b(+)

Data sets:
Data typeCount
13C chemical shifts679
15N chemical shifts178
1H chemical shifts1110

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Pyrazinamidase1

Entities:

Entity 1, Pyrazinamidase 186 residues - 19604.6 Da.

1   METARGALALEUILEILEVALASPVALGLN
2   ASNASPPHECYSGLUGLYGLYSERLEUALA
3   VALTHRGLYGLYALAALALEUALAARGALA
4   ILESERASPTYRLEUALAGLUALAALAASP
5   TYRHISHISVALVALALATHRLYSASPPHE
6   HISILEASPPROGLYASPHISPHESERGLY
7   THRPROASPTYRSERSERSERTRPPROPRO
8   HISCYSVALSERGLYTHRPROGLYALAASP
9   PHEHISPROSERLEUASPTHRSERALAILE
10   GLUALAVALPHETYRLYSGLYALATYRTHR
11   GLYALATYRSERGLYPHEGLUGLYVALASP
12   GLUASNGLYTHRPROLEULEUASNTRPLEU
13   ARGGLNARGGLYVALASPGLUVALASPVAL
14   VALGLYILEALATHRASPHISCYSVALARG
15   GLNTHRALAGLUASPALAVALARGASNGLY
16   LEUALATHRARGVALLEUVALASPLEUTHR
17   ALAGLYVALSERALAASPTHRTHRVALALA
18   ALALEUGLUGLUMETARGTHRALASERVAL
19   GLULEUVALCYSSERSER

Samples:

sample_1: Pyrazinamidase, [U-13C; U-15N; 70%-2H], 0.8 ± 0.1 mM; DTT 10 mM; NaN3 1 mM

sample_2: Pyrazinamidase, [U-13C; U-15N], 0.8 ± 0.1 mM; DTT 10 mM; NaN3 1 mM

sample_3: Pyrazinamidase, [U-15N], 0.8 ± 0.1 mM; DTT 10 mM; NaN3 1 mM

conditions_1: pH: 6.7; temperature: 313 K

Experiments:

NameSampleSample stateSample conditions
1H15N_HSQCnot availablenot availableconditions_1
1H15N_TROSYnot availablenot availableconditions_1
3D TROSY-HNCAnot availablenot availableconditions_1
3D TROSY-HN(CO)CAnot availablenot availableconditions_1
3D TROSY-HNCACBnot availablenot availableconditions_1
3D TROSY-HN(CO)CACBnot availablenot availableconditions_1
3D TROSY-HN(CA)COnot availablenot availableconditions_1
3D TROSY-HNCOnot availablenot availableconditions_1
3D CC(CO)NHnot availablenot availableconditions_1
3D (H)CC(CO)NHnot availablenot availableconditions_1
3D HNHAnot availablenot availableconditions_1
3D HBHA(CO)NHnot availablenot availableconditions_1
3D 15N-edited NOESY-TROSYnot availablenot availableconditions_1
3D 13C-edited NOESY-HSQCnot availablenot availableconditions_1
3D 13C-edited TOCSY-HSQCnot availablenot availableconditions_1
3D HCCH-COSYnot availablenot availableconditions_1
3D HCCH-TOCSYnot availablenot availableconditions_1

Software:

No software information available

NMR spectrometers:

  • Bruker Avance 600 MHz
  • Bruker Avance 800 MHz
  • Bruker Avance 500 MHz

Related Database Links:

PDB
DBJ BAH26342 BAL66049 BAO48146 BAQ06101 GAA45748
EMBL CAD96922 CAL72050 CCC27128 CCC44398 CCC64640
GB AAB37768 AAK38743 AAK46382 AAV33179 AAV33180
REF NP_216559 NP_336568 NP_855719 WP_003410243 WP_003906725

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks