BMRB Entry 7010

Title:
Solution Structure and Immune Response to an Epidermal Growth Factor Domain from Plasmodium Falciparum Merozoite Surface Protein 1
Deposition date:
2006-02-28
Original release date:
2007-11-16
Authors:
James, S.; Moehle, K.; Pluschke, G.; Robinson, J.
Citation:

Citation: James, S.; Moehle, K.; Renard, A.; Mueller, M.; Vogel, D.; Zurbriggen, R.; Pluschke, G.; Robinson, J.. "Synthesis, Solution Structure and Immune Recognition of an Epidermal Growth Factor-Like Domain from Plasmodium falciparum Merozoite Surface Protein-1"  ChemBioChem 7, 1943-1950 (2006).
PubMed: 17068840

Assembly members:

Assembly members:
Merozoite surface protein 1, polymer, 49 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: malaria parasite P. falciparum   Taxonomy ID: 5833   Superkingdom: Eukaryota   Kingdom: not available   Genus/species: Plasmodium falciparum

Experimental source:

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Merozoite surface protein 1: NISQHQCVKKQCPQNSGCFR HLDEREECKCLLNYKQEGDK CVENPNPTX

Data sets:
Data typeCount
1H chemical shifts577

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Merozoite surface protein 11

Entities:

Entity 1, Merozoite surface protein 1 49 residues - Formula weight is not available

1   ASNILESERGLNHISGLNCYSVALLYSLYS
2   GLNCYSPROGLNASNSERGLYCYSPHEARG
3   HISLEUASPGLUARGGLUGLUCYSLYSCYS
4   LEULEUASNTYRLYSGLNGLUGLYASPLYS
5   CYSVALGLUASNPROASNPROTHRNH2

Samples:

sample_1: Merozoite surface protein 1 1.25 mM; H2O 90%; D2O 10%

sample_2: Merozoite surface protein 1 1.25 mM; D2O 100%

sample_cond_1: ionic strength: 0 mM; pH: 5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D NOESYnot availablenot availablesample_cond_1
2D TOCSYnot availablenot availablesample_cond_1
DQF-COSYnot availablenot availablesample_cond_1

Software:

xwinnmr v2.6 - processing

XEASY v1.53 - data analysis

CYANA v2.1 - refinement, structure solution

NMR spectrometers:

  • Bruker DRX 600 MHz