BMRB Entry 6989

Title:
1H, 13C, and 15N Chemical Shift Assignments for Ribosomal Protein S24E
Deposition date:
2006-02-15
Original release date:
2007-03-06
Authors:
Jeon, Byoung-Young; Hong, Enn-mi; Jung, Jin-won; Yee, Adelinda; Cheryl H, Arrowsmith; Lee, Weontae
Citation:

Citation: Ban, N.; Nissen, P.; Hansen, J.; Capel, M.; Moore, Peter; Steitz, T.. "Placement of protein and RNA structures into a 5 A-resolution map of the 50S ribosomal subunit."  Nature 400, 841-847 (1999).
PubMed: 10476961

Assembly members:

Assembly members:
Ribosomal Protein S24E, polymer, 98 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Thermoplasma acidophilum   Taxonomy ID: 2303   Superkingdom: Archaea   Kingdom: not available   Genus/species: Thermoplasma acidophilum

Experimental source:

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts404
15N chemical shifts92
1H chemical shifts603

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Ribosomal Protein S24E1

Entities:

Entity 1, Ribosomal Protein S24E 98 residues - Formula weight is not available

1   METASPLEUILEILELYSGLULYSARGASP
2   ASNPROILELEULYSARGLYSGLUILELYS
3   TYRVALLEULYSPHEASPSERSERARGTHR
4   PROSERARGGLUGLUILELYSGLULEUILE
5   ALALYSHISGLUGLYVALASPLYSGLULEU
6   VALILEVALASPASNASNLYSGLNLEUTHR
7   GLYLYSHISGLUILEGLUGLYTYRTHRLYS
8   ILETYRALAASPLYSPROSERALAMETLEU
9   TYRGLUPROASPTYRGLULEUILEARGASN
10   GLYLEULYSGLNLYSGLUALALYS

Samples:

sample_1: Ribosomal Protein S24E, [U-13C; U-15N], 1.5 mM; photasuim phosphate 50 mM; NaN3 0.1 mM; H2O 90%; D2O 10%

sample_2: Ribosomal Protein S24E, [U-15N], 1.5 mM; photasuim phosphate 50 mM; NaN3 0.1 mM; H2O 90%; D2O 10%

sample_3: Ribosomal Protein S24E, [U-13C], 1.5 mM; photasuim phosphate 50 mM; NaN3 0.1 mM; D2O 100%

conditions_1: pH: 6.5; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
1H15N_HSQCsample_2not availableconditions_1
HNCAsample_2not availableconditions_1
HNCACBsample_1not availableconditions_1
CBCA(CO)NHsample_1not availableconditions_1
H(CC)(CO)NHsample_1not availableconditions_1
HCCONHsample_1not availableconditions_1
3D_15N-edited_NOESYsample_2not availableconditions_1
3D_13C-edited_NOESYsample_3not availableconditions_1

Software:

No software information available

NMR spectrometers:

  • Bruker DRX 500 MHz
  • Varian INOVA 500 MHz
  • Bruker DRX 800 MHz

Related Database Links:

PDB
EMBL CAC12220
REF NP_394551 WP_010901502
SP Q9HJ79
AlphaFold Q9HJ79

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks