BMRB Entry 6979

Title:
An Alternating Sheared AA Pair and Elements of Stability for a Single Sheared Purine-Purine Pair Flanked by Sheared GA Pairs
Deposition date:
2006-02-09
Original release date:
2006-06-26
Authors:
Chen, G.; Kennedy, S.; Krugh, T.; Turner, D.
Citation:

Citation: Chen, Gang; Kennedy, Scott; Qiao, Jing; Krugh, Thomas; Turner, Douglas. "An Alternating Sheared AA Pair and Elements of Stability for a Single Sheared Purine-Purine Pair Flanked by Sheared GA Pairs in RNA"  Biochemistry 45, 6889-6903 (2006).

Assembly members:

Assembly members:
GGUGAAGGCU, polymer, 10 residues, Formula weight is not available
GCCGAAGCCP, polymer, 10 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: not available   Taxonomy ID: not available   Superkingdom: not available   Kingdom: not available   Genus/species: not available not available

Experimental source:

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
GGUGAAGGCU: GGUGAAGGCU
GCCGAAGCCP: GCCGAAGCCX

Data sets:
Data typeCount
1H chemical shifts168
31P chemical shifts18

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
15'-R(*GP*GP*UP*GP*AP*AP*GP*GP*CP*U)-3'1
25'-R(*GP*CP*CP*GP*AP*AP*GP*CP*CP*(PR5))-3'2

Entities:

Entity 1, 5'-R(*GP*GP*UP*GP*AP*AP*GP*GP*CP*U)-3' 10 residues - Formula weight is not available

1   GGUGAAGGCU

Entity 2, 5'-R(*GP*CP*CP*GP*AP*AP*GP*CP*CP*(PR5))-3' 10 residues - Formula weight is not available

1   GCCGAAGCCP5P

Samples:

sample_1: GGUGAAGGCU 2 mM; GCCGAAGCCP 2 mM; NaCl 80 mM; sodium phosphate 10 mM; Na2EDTA 0.5 mM

sample_cond_1: pH: 5.1; temperature: 303 K

sample_cond_2: pH: 5.1; temperature: 278 K

Experiments:

NameSampleSample stateSample conditions
not availablesample_1not availablenot available

Software:

FELIX -

DISCOVER -

VNMR -

NMR spectrometers:

  • Varian INOVA 600 MHz
  • Varian INOVA 500 MHz