BMRB Entry 6850

Title:
First FF domain of the PRP40 yeast protein
Deposition date:
2005-10-05
Original release date:
2007-01-29
Authors:
Gasch, Alexander; Wiesner, Silke; Martin-Malpartida, Pau; Ramirez-Espain, Ximena; Ruiz, Lidia; Macias, Maria
Citation:

Citation: Gasch, Alexander; Wiesner, Silke; Martin-Malpartida, Pau; Ramirez-Espain, Ximena; Ruiz, Lidia; Macias, Maria. "The structure of Prp40 FF1 domain and its interaction with the crn-TPR1 motif of CLF1 gives a new insight into the binding mode of FF domains."  J. Biol. Chem. 281, 356-364 (2006).
PubMed: 16253993

Assembly members:

Assembly members:
PRP40 first FF domain, polymer, 59 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Baker's Yeast   Taxonomy ID: 4932   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Saccharomyces cerevisiae

Experimental source:

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
PRP40 first FF domain: GAMEAEKEFITMLKENQVDS TWSFSRIISELGTRDPRYWM VDDDPLWKKEMFEKYLSNR

Data sets:
Data typeCount
13C chemical shifts209
15N chemical shifts65
1H chemical shifts387

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1First FF domain of Prp401

Entities:

Entity 1, First FF domain of Prp40 59 residues - Formula weight is not available

1   GLYALAMETGLUALAGLULYSGLUPHEILE
2   THRMETLEULYSGLUASNGLNVALASPSER
3   THRTRPSERPHESERARGILEILESERGLU
4   LEUGLYTHRARGASPPROARGTYRTRPMET
5   VALASPASPASPPROLEUTRPLYSLYSGLU
6   METPHEGLULYSTYRLEUSERASNARG

Samples:

15N: PRP40 first FF domain, [U-15N], 1 mM; Na Phosphate 20 mM; NaCl 100 mM; H2O 90%; D2O 10%

13C: PRP40 first FF domain, [U-13C; U-15N], 1 mM; Na Phosphate 20 mM; NaCl 100 mM; H2O 90%; D2O 10%

13CD2O: PRP40 first FF domain, [U-13C; U-15N], 1 mM; Na Phosphate 20 mM; NaCl 100 mM; D2O 100%

conditions_1: pressure: 1 atm; temperature: 285 K

Experiments:

NameSampleSample stateSample conditions
15N edited NOESYnot availablenot availableconditions_1
13C edited NOESYnot availablenot availableconditions_1

Software:

xwinnmr v3.6, Bruker Biospin AG - Acquisition

NMRPipe, Delaglio F. - processing

CNS v1.1, Brunger A.T. -

NMR spectrometers:

  • Bruker DRX 600 MHz

Related Database Links:

PDB
DBJ GAA24712
EMBL CAA81847 CAY81071
GB AAB24902 AHY76231 EDN59896 EDV13075 EDZ70922
REF NP_012913
SP P33203
TPG DAA09144
AlphaFold P33203

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks