BMRB Entry 6649

Title:
Solution structure of the peptidoglycan binding domain of B. subtilis cell wall lytic enzyme CwlC: Characterization of the sporulation-related repeats by NMR
Deposition date:
2005-05-26
Original release date:
2005-12-05
Authors:
Mishima, M.; Shida, T.; Yabuki, K.; Kato, K.; Sekiguchi, J.; Kojima, C.
Citation:

Citation: Mishima, M.; Shida, T.; Yabuki, K.; Kato, K.; Sekiguchi, J.; Kojima, C.. "Solution structure of the peptidoglycan binding domain of Bacillus subtilis cell wall lytic enzyme CwlC: characterization of the sporulation-related repeats by NMR."  Biochemistry 44, 10153-10163 (2005).
PubMed: 16042392

Assembly members:

Assembly members:
Sporulation-specific N-acetylmuramoyl-L-alanine amidase (E.C.3.5.1.28), polymer, 79 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Bacillus subtilis   Taxonomy ID: 1423   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Bacillus subtilis

Experimental source:

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Sporulation-specific N-acetylmuramoyl-L-alanine amidase (E.C.3.5.1.28): LKKTSSSGLYKVQIGAFKVK ANADSLASNAEAKGFDSIVL LKDGLYKVQIGAFSSKDNAD TLAARAKNAGFDAIVILES

Data sets:
Data typeCount
1H chemical shifts444
13C chemical shifts212
15N chemical shifts72

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Sporulation-specific N-acetylmuramoyl-L-alanine amidase1

Entities:

Entity 1, Sporulation-specific N-acetylmuramoyl-L-alanine amidase 79 residues - Formula weight is not available

1   LEULYSLYSTHRSERSERSERGLYLEUTYR
2   LYSVALGLNILEGLYALAPHELYSVALLYS
3   ALAASNALAASPSERLEUALASERASNALA
4   GLUALALYSGLYPHEASPSERILEVALLEU
5   LEULYSASPGLYLEUTYRLYSVALGLNILE
6   GLYALAPHESERSERLYSASPASNALAASP
7   THRLEUALAALAARGALALYSASNALAGLY
8   PHEASPALAILEVALILELEUGLUSER

Samples:

sample_1: Sporulation-specific N-acetylmuramoyl-L-alanine amidase (E.C.3.5.1.28), [U-15N; U-13C], 1.7 mM; phosphate buffer K 50 mM; D2O 100%

sample_2: Sporulation-specific N-acetylmuramoyl-L-alanine amidase (E.C.3.5.1.28), [U-15N], 1.7 mM; phosphate buffer K 50 mM; H2O 90%; D2O 10%

sample_cond_1: pH: 6.9; temperature: 303 K; ionic strength: 70 mM; pressure: 1 atm

Experiments:

NameSampleSample stateSample conditions
3D 13C-separated NOESYnot availablenot availablenot available
3D 15N-separated NOESYnot availablenot availablenot available

Software:

NMRPipe vver 2.3 - processing

Sparky vver 3.110 - data analysis

CYANA vver 1.05 - structure solution

CNS vver 1.1 - refinement

NMR spectrometers:

  • Bruker AVANCE 500 MHz

Related Database Links:

PDB
DBJ BAA03500 BAM52388 BAM57964
EMBL CAA92813 CAB13625 CCU58317 CEI56925 CEJ77332
GB AEP90898 AFQ57673 AGA23958 AGE63582 AGG61112
REF NP_389623 WP_003244862 WP_014476879 WP_015252039 WP_015383726
SP Q06320
AlphaFold Q06320

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks