BMRB Entry 6596

Title:
NMR solution structure of a leaf-specific-expressed cyclotide vhl-1
Deposition date:
2005-04-14
Original release date:
2005-08-22
Authors:
Chen, B.; Colgrave, M.; Daly, N.; Rosengren, K.; Gustafson, K.; Craik, D.
Citation:

Citation: Chen, B.; Colgrave, M.; Daly, N.; Rosengren, K.; Gustafson, K.; Craik, D.. "Isolation and characterisation of novel cyclotides from Viola hederaceae: solution structure and anti-HIV activity of vhl-1, a leaf-specific-expressed cyclotide"  J. Biol. Chem. 280, 22395-22405 (2005).
PubMed: 15824119

Assembly members:

Assembly members:
vhl-1, polymer, 31 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Australian violet   Taxonomy ID: 180952   Superkingdom: Eukaryota   Kingdom: Viridiplantae   Genus/species: Viola hederacea

Experimental source:

Experimental source:   Production method: .

Entity Sequences (FASTA):

Entity Sequences (FASTA):
vhl-1: CGESCAMISFCFTEVIGCSC KNKVCYLNSIS

Data sets:
Data typeCount
1H chemical shifts200

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1vhl-11

Entities:

Entity 1, vhl-1 31 residues - Formula weight is not available

1   CYSGLYGLUSERCYSALAMETILESERPHE
2   CYSPHETHRGLUVALILEGLYCYSSERCYS
3   LYSASNLYSVALCYSTYRLEUASNSERILE
4   SER

Samples:

sample_1: vhl-1 1.4 mM; H2O 70%; CD3CN 25%; D2O 5%

sample_cond_1: ionic strength: 0 M; pH: 3.3; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D NOESYsample_1not availablesample_cond_1
2D TOCSYsample_1not availablesample_cond_1
DQF-COSYsample_1not availablesample_cond_1
E-COSYsample_1not availablesample_cond_1

Software:

xwinnmr v3.5 - collection

SPARKY v3.110 - data analysis

DYANA v1.5 - iterative matrix relaxation

CNS v1.1 - refinement

NMR spectrometers:

  • Bruker AVANCE 600 MHz

Related Database Links:

PDB
SWISS-PROT P84522