BMRB Entry 6594

Title:
Elucidation of the high-resolution solution structure of a trypanosomatid FYVE domain
Deposition date:
2005-04-14
Original release date:
2007-11-14
Authors:
Mertens, H.; Callaghan, J.; McConville, M.; Gooley, P.
Citation:

Citation: Mertens, H.; Callaghan, J.; Swarbrick, J.; McConville, M.; Gooley, P.. "A high-resolution solution structure of a trypanosomatid FYVE domain"  Protein Sci. 16, 2552-2559 (2007).

Assembly members:

Assembly members:
Leishmania major FYVE domain containing protein 1, polymer, 84 residues, Formula weight is not available
ZN, non-polymer, 65.409 Da.

Natural source:

Natural source:   Common Name: Leishmania major   Taxonomy ID: 5664   Superkingdom: Eukaryota   Kingdom: not available   Genus/species: Leishmania major

Experimental source:

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Leishmania major FYVE domain containing protein 1: GPLGSMGEKQSKGYWQEDED APACNGCGCVFTTTVRRHHC RNCGYVLCGDCSRHRAAIPM RGITEPERVCDACYLALRSS NMAG

Data sets:
Data typeCount
13C chemical shifts323
15N chemical shifts87
1H chemical shifts492

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1LM5-11
2ZINC (II) ION, 12
3ZINC (II) ION, 22

Entities:

Entity 1, LM5-1 84 residues - Formula weight is not available

1   GLYPROLEUGLYSERMETGLYGLULYSGLN
2   SERLYSGLYTYRTRPGLNGLUASPGLUASP
3   ALAPROALACYSASNGLYCYSGLYCYSVAL
4   PHETHRTHRTHRVALARGARGHISHISCYS
5   ARGASNCYSGLYTYRVALLEUCYSGLYASP
6   CYSSERARGHISARGALAALAILEPROMET
7   ARGGLYILETHRGLUPROGLUARGVALCYS
8   ASPALACYSTYRLEUALALEUARGSERSER
9   ASNMETALAGLY

Entity 2, ZINC (II) ION, 1 - Zn - 65.409 Da.

1   ZN

Samples:

sample_1: Leishmania major FYVE domain containing protein 1, [U-15N; U-13C], 0.8 mM; sodium phosphate buffer 50 mM; KCl 50 mM; DTT 1 mM; AEBSF 1 mM; sodium azide 0.05%; H2O 90%; D2O 10%

sample_2: Leishmania major FYVE domain containing protein 1, [U-15N; U-13C], 0.8 mM; sodium phosphate buffer 50 mM; KCl 50 mM; DTT 1 mM; AEBSF 1 mM; sodium azide 0.05%; D2O 99.99%

sample_3: Leishmania major FYVE domain containing protein 1, [U-10% 13C], 0.8 mM; sodium phosphate buffer 50 mM; KCl 50 mM; DTT 1 mM; AEBSF 1 mM; sodium azide 0.05%; H2O 90%; D2O 10%

sample_cond_1: pH: 7.4; pressure: 1 atm; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
3D 13C-separated NOESYnot availablenot availablesample_cond_1
3D 15N-separated NOESYnot availablenot availablesample_cond_1
HNHAnot availablenot availablesample_cond_1
2D 13C-ct-HSQCnot availablenot availablesample_cond_1

Software:

VNMR v6.1c - collection

NMRPipe v2.2 - processing

XEASY v1.3 - data analysis

CYANA v1.0.7 - structure solution

X-PLOR NIH v2.9.1 - refinement

NMR spectrometers:

  • Varian INOVA 600 MHz

Related Database Links:

PDB

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks