BMRB Entry 6554

Title:
Experimental evidence for a reversible alfa to beta transition of Abeta-(1-42) in aqueous media: a step by step conformational analysis pinpoints the location of the beta conformation seeding
Deposition date:
2005-03-18
Original release date:
2006-04-17
Authors:
Tomaselli, S.; Esposito, V.; Vangone, P.; van Nuland, N.; Bonvin, A.; Guerrini, R.; Tancredi, T.; Temussi, P.; Picone, D.
Citation:

Citation: Tomaselli, S.; Esposito, V.; Vangone, P.; van Nuland, N.; Bonvin, A.; Guerrini, R.; Tancredi, T.; Temussi, P.; Picone, D.. "The alpha-to-beta Conformational Transition of Alzheimer's Abeta-(1-42) Peptide in Aqueous Media is Reversible: A Step by Step Conformational Analysis Suggests the Location of beta Conformation Seeding."  Chembiochem. 7, 257-267 (2006).
PubMed: 16444756

Assembly members:

Assembly members:
Alzheimer's disease amyloid, polymer, 42 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Alzheimer's disease amyloid: DAEFRHDSGYEVHHQKLVFF AEDVGSNKGAIIGLMVGGVV IA

Data sets:
Data typeCount
13C chemical shifts30
1H chemical shifts254

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Alzheimer's disease amyloid1

Entities:

Entity 1, Alzheimer's disease amyloid 42 residues - Formula weight is not available

1   ASPALAGLUPHEARGHISASPSERGLYTYR
2   GLUVALHISHISGLNLYSLEUVALPHEPHE
3   ALAGLUASPVALGLYSERASNLYSGLYALA
4   ILEILEGLYLEUMETVALGLYGLYVALVAL
5   ILEALA

Samples:

sample_1: Alzheimer's disease amyloid 2 mM; hexafluoroisopropanol-d2 30%; H2O 70%

sample_cond_1: pressure: 1 atm; temperature: 300 K

Experiments:

NameSampleSample stateSample conditions
2D TOCSYnot availablenot availablenot available
2D NOESYnot availablenot availablenot available
13C-HSQCnot availablenot availablenot available

Software:

NMRPipe v1.5 - processing

DYANA v1.5 - refinement, structure solution

XWINNMR v3.0 - collection

nmrview v5.0.4 - data analysis

NMR spectrometers:

  • Bruker AVANCE 750 MHz
  • Bruker . 600 MHz
  • Bruker . 500 MHz
  • Bruker . 400 MHz

Related Database Links:

BMRB 5400 6257
PDB
DBJ BAA22264 BAA84580 BAD51938 BAE01907 BAG10647
EMBL CAA30050 CAA31830 CAA39589 CAA39590 CAA39591
GenBank AAA35540 AAA36829 AAA51564 AAA51722 AAA51726
PIR A60045 D60045 E60045 G60045 PQ0438
PRF 1303338A 1403400A 1405204A 1507304A 1507304B
REF NP_000475 NP_001006601 NP_001013036 NP_001070264 NP_958816
SWISS-PROT P05067 P53601 P79307 Q28053 Q28280