BMRB Entry 6527

Title:
1H, 13C and 15N Chemical Shift Assignments for km23 dimer
Deposition date:
2005-03-01
Original release date:
2005-11-01
Authors:
Ilangovan, Udayar; Ding, Wei; Groppe, Jay; Trbovich, James; Zuniga, Jorge; Demeler, Borries; Mulder, Kathleen; Hinck, Andrew
Citation:

Citation: Ilangovan, Udayar; Ding, Wei; Zhong, Yan; Wilson, Christina; Groppe, Jay; Trbovich, James; Zuniga, Jorge; Demeler, Borries; Tang, Qian; Gao, Guofeng; Mulder, Kathleen; Hinck, Andrew. "Structure and Dynamics of the Homodimeric Dynein Light Chain km23."  J. Mol. Biol. 352, 338-354 (2005).
PubMed: 16083906

Assembly members:

Assembly members:
Homedimeric Km23, polymer, 96 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts442
15N chemical shifts100
1H chemical shifts576
coupling constants65

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Km23 homodimer unit one1
2Km23 homodimer unit two1

Entities:

Entity 1, Km23 homodimer unit one 96 residues - Formula weight is not available

1   METALAGLUVALGLUGLUTHRLEULYSARG
2   LEUGLNSERGLNLYSGLYVALGLNGLYILE
3   ILEVALVALASNTHRGLUGLYILEPROILE
4   LYSSERTHRMETASPASNPROTHRTHRTHR
5   GLNTYRALASERLEUMETHISSERPHEILE
6   LEULYSALAARGSERTHRVALARGASPILE
7   ASPPROGLNASNASPLEUTHRPHELEUARG
8   ILEARGSERLYSLYSASNGLUILEMETVAL
9   ALAPROASPLYSASPTYRPHELEUILEVAL
10   ILEGLNASNPROTHRGLU

Samples:

sample_1: Homedimeric Km23, [U-15N], 1 mM

sample_2: Homedimeric Km23, [U-13 C; U-15N], 1 mM

conditions_1: pH: 6.0; temperature: 315 K

Experiments:

NameSampleSample stateSample conditions
1H15N_HSQCnot availablenot availablenot available
HNCACBnot availablenot availablenot available
CBCA(CO)NHnot availablenot availablenot available
15N_NOESYnot availablenot availablenot available
13C_NOESYnot availablenot availablenot available

Software:

NMRPipe -

NMRView -

NMR spectrometers:

  • Bruker Avance 600 MHz

Related Database Links:

BMRB 15142 6210
PDB
DBJ BAG57523
GB AAF29126 AAF86646 AAH02481 AAK18712 AAK95342
REF NP_001177109 NP_001268656 NP_001268657 NP_001268658 NP_054902
SP Q9NP97
AlphaFold Q9NP97

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks