BMRB Entry 6272

Title:
NMR ASSIGNMENT OF MTH0776 FROM METHANOBACTERIUM THERMOAUTOTROPHICUM STRAIN H
Deposition date:
2004-07-23
Original release date:
2005-10-28
Authors:
AMEGBEY, GODWIN; ZHAN, CHANG; YEE, ADELINDA; ARROWSMITH, CHERYL; WISHART, DAVID
Citation:

Citation: AMEGBEY, GODWIN; CHANG, ZHAN; STOTHARD, PAUL; YEE, ADELINDA; ARROWSMITH, CHERYL; WISHART, DAVID. "Letter to the Editor: 1H, 13C AND 15N NMR Assignments of MTH0776 from Methanobacterium thermoautotrophicum"  J. Biomol. NMR 30, 459-460 (2004).

Assembly members:

Assembly members:
MTH0776, polymer, 121 residues, 11397.37 Da.

Natural source:

Natural source:   Common Name: Methanobacterium thermoautotrophicum   Taxonomy ID: 145262   Superkingdom: Archaea   Kingdom: not available   Genus/species: Methanothermobacter thermautotrophicus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Data sets:
Data typeCount
1H chemical shifts829
13C chemical shifts463
15N chemical shifts110

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Conserved protein from methanobacterium thermoautotrophicum1

Entities:

Entity 1, Conserved protein from methanobacterium thermoautotrophicum 121 residues - 11397.37 Da.

1   METGLYSERSERHISHISHISHISHISHIS
2   SERSERGLYLEUVALPROARGGLYSERHIS
3   METTHRPHECYSLEUGLUTHRTYRLEUGLN
4   GLNSERGLYGLUTYRGLUILEHISMETLYS
5   ARGALAGLYPHEARGGLUCYSALAALAMET
6   ILEGLULYSLYSALAARGARGVALVALHIS
7   ILELYSPROGLYGLULYSILELEUGLYALA
8   ARGILEILEGLYILEPROPROVALPROILE
9   GLYILEASPGLUGLUARGSERTHRVALMET
10   ILEPROTYRTHRLYSPROCYSTYRGLYTHR
11   ALAVALVALGLULEUPROVALASPPROGLU
12   GLUILEGLUARGILELEUGLUVALALAGLU
13   PRO

Samples:

sample: MTH0776, [U-100% 13C; U-100% 15N], 1 mM; NaH2PO4 50 mM; NaCL 300 mM; DTT 15 mM; NaN3 0.06%; DSS 0.1 mM

sample_conditions: pH: 6.8; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 15N HSQCsamplenot availablesample_conditions
2D 13C HSQCsamplenot availablesample_conditions
2D 1H-1H NOESYsamplenot availablesample_conditions
3D HNCACBsamplenot availablesample_conditions
HNCAsamplenot availablesample_conditions
CBCA (CO) NHsamplenot availablesample_conditions
HNCOsamplenot availablesample_conditions
HNHAsamplenot availablesample_conditions
3D H (CCO) NHsamplenot availablesample_conditions
C (CO) NHsamplenot availablesample_conditions
DE-H (C) CH-TOCSYsamplenot availablesample_conditions
3D 1H-15N NOESYsamplenot availablesample_conditions
13C NOESYsamplenot availablesample_conditions

Software:

No software information available

NMR spectrometers:

  • VARIAN INOVA 500 MHz
  • VARIAN INOVA 800 MHz

Related Database Links:

PDB
DBJ BAM69944
GB AAB85279
REF NP_275918 WP_010876414

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks