BMRB Entry 6037

Title:
Solution Structure of BmKX, a novel potassium channel blocker from the Chinese Scorpion Buthus martensi Karsch
Deposition date:
2003-12-19
Original release date:
2005-02-21
Authors:
Cai, Z.; Wu, J.; Xu, Y.; Wang, C.; Chi, C.-W.; Shi, Y.
Citation:

Citation: Wang, C.; Cai, Z.; Lu, W.; Wu, J.; Xu, Y.; Shi, Y.; Chi, C.-W.. "A novel short-chain peptide BmKX from the chinese scorpion Buthus martensi karsch, sequencing, gene cloning and structure determination"  Toxicon 45, 309-319 (2005).
PubMed: 15683869

Assembly members:

Assembly members:
BmKX, polymer, 31 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Chinese scorpion   Taxonomy ID: 34649   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mesobuthus martensii

Experimental source:

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
BmKX: TPYPVNCKTDRDCVMCGLGI SCKNGYCQGCT

Data sets:
Data typeCount
1H chemical shifts183

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1potassium channel toxin KX1

Entities:

Entity 1, potassium channel toxin KX 31 residues - Formula weight is not available

1   THRPROTYRPROVALASNCYSLYSTHRASP
2   ARGASPCYSVALMETCYSGLYLEUGLYILE
3   SERCYSLYSASNGLYTYRCYSGLNGLYCYS
4   THR

Samples:

sample_1: BmKX 3 mM; Phosphate buffer 50 mM; H2O 90%; D2O 10%

sample_2: BmKX 3 mM; Phosphate buffer 50 mM; D2O 99.96%

sample_cond_1: ionic strength: 50 mM; pH: 5.0; pressure: 1 atm; temperature: 300 K

Experiments:

NameSampleSample stateSample conditions
DQF-COSYnot availablenot availablenot available
2D TOCSYnot availablenot availablenot available
2D NOESYnot availablenot availablenot available
E-COSYnot availablenot availablenot available

Software:

XEasy v3.2 - data analysis

DIANA v2.8 - data analysis

CNS v1.0 - structure solution, refinement

NMR spectrometers:

  • Bruker DMX 500 MHz

Related Database Links:

PDB
GenBank AAM91031 AAN39841
SWISS-PROT Q7Z0H4