BMRB Entry 6000

Title:
1H, 13C and 15N Resonance Assignments of the Drosophila Argonaute1 PAZ Domain
Deposition date:
2003-11-07
Original release date:
2005-01-20
Authors:
Yan, Kelley; Farooq, Amjad; Zeng, Lei; Han, Arnold; Yan, Sherry; Zhou, Ming-Ming
Citation:

Citation: Yan, Kelley; Yan, Sherry; Farooq, Amjad; Han, Arnold; Zeng, Lei; Zhou, Ming-Ming. "Structure and conserved RNA binding of the PAZ domain"  Nature 426, 468-474 (2003).
PubMed: 14615802

Assembly members:

Assembly members:
Drosophila Argonaute1 PAZ domain, polymer, 169 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: fruit fly   Taxonomy ID: 7227   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Drosophila melanogaster

Experimental source:

Experimental source:   Production method: recombinant technology

Data sets:
Data typeCount
1H chemical shifts1026
13C chemical shifts515
15N chemical shifts152

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Ago1 PAZ domain1

Entities:

Entity 1, Ago1 PAZ domain 169 residues - Formula weight is not available

1   GLYTHRALAPHETYRLYSALAGLNPROVAL
2   ILEASPPHEMETCYSGLUVALLEUASPILE
3   ARGASPILEASNGLUGLNARGLYSPROLEU
4   THRASPSERGLNARGVALLYSPHETHRLYS
5   GLUILELYSGLYLEULYSILEGLUILETHR
6   HISCYSGLYGLNMETARGARGLYSTYRARG
7   VALCYSASNVALTHRARGARGPROALAGLN
8   METGLNSERPHEPROLEUGLNLEUGLUASN
9   GLYGLNTHRVALGLUCYSTHRVALALALYS
10   TYRPHELEUASPLYSTYRARGMETLYSLEU
11   ARGTYRPROHISLEUPROCYSLEUGLNVAL
12   GLYGLNGLUHISLYSHISTHRTYRLEUPRO
13   LEUGLUVALCYSASNILEVALALAGLYGLN
14   ARGCYSILELYSLYSLEUTHRASPMETGLN
15   THRSERTHRMETILELYSALATHRALAARG
16   SERALAPROASPARGGLUARGGLUILEASN
17   ASNLEUVALLYSARGALAASPPHEASN

Samples:

sample_1: Drosophila Argonaute1 PAZ domain, [U-15N], 0.5 mM; H20 100%

sample_2: Drosophila Argonaute1 PAZ domain, [U-15N; U-13C], 0.5 mM; D20 100%

sample_3: Drosophila Argonaute1 PAZ domain, [U-15N; U-13C], 0.5 mM; H20 100%

cond_1: pH: 6.5; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
not availablenot availablenot availablecond_1

Software:

No software information available

NMR spectrometers:

  • . . 500 MHz
  • . . 600 MHz

Related Database Links:

PDB
DBJ BAA88078
GB AAF58313 AAF58314 AAF58315 AAK93297 ABB36449
REF NP_001246314 NP_523734 NP_725341 NP_725342 XP_001360946

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks