BMRB Entry 5749

Title:
Isolation, gene expression and solution structure of a novel moricin analogue, an antibacterial peptide from a lepidopteran insect, Spodoptera litura
Deposition date:
2003-03-20
Original release date:
2005-09-08
Authors:
Oizumi, Yuki; Minami, Masayoshi; Hemmi, Hikaru; Asaoka, Ai; Yamakawa, Minoru
Citation:

Citation: Oizumi, Yuki; Hemmi, Hikaru; Minami, Masayoshi; Asaoka, Ai; Yamakawa, Minoru. "Isolation, gene expression and solution structure of a novel moricin analogue, an antibacterial peptide from a lepidopteran insect, Spodoptera litura"  Biochim. Biophys. Acta 1752, 83-92 (2005).
PubMed: 16115804

Assembly members:

Assembly members:
Sl-moricin, polymer, 42 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Spodoptera litura   Taxonomy ID: 69820   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Spodoptera litura

Experimental source:

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Sl-moricin: GKIPVKAIKKAGAAIGKGLR AINIASTAHDVYSFFKPKHK KK

Data sets:
Data typeCount
1H chemical shifts315

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Sl-moricin monomer1

Entities:

Entity 1, Sl-moricin monomer 42 residues - Formula weight is not available

1   GLYLYSILEPROVALLYSALAILELYSLYS
2   ALAGLYALAALAILEGLYLYSGLYLEUARG
3   ALAILEASNILEALASERTHRALAHISASP
4   VALTYRSERPHEPHELYSPROLYSHISLYS
5   LYSLYS

Samples:

sample_1: Sl-moricin mM; CD3OH 100%

Ex-cond_1: pH: 2.4; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
1H-1H TOCSYnot availablenot availablenot available
1H-1H DQF-COSYnot availablenot availablenot available
1H-1H NOESYnot availablenot availablenot available

Software:

xwinnmr v2.6 - acquisition and processing

SPARKY v3.1 - peak assignment

NMR spectrometers:

  • Avance Bruker 800 MHz
  • Avance Bruker 500 MHz

Related Database Links:

PDB
DBJ BAC79440
GB AAT38873