BMRB Entry 5409

Title:
Backbone Chemical Shift Assignments for 2H, 13C, 15N-labeled Human PTB1 Protein
Deposition date:
2002-06-28
Original release date:
2002-08-22
Authors:
Simpson, Peter; Natalia, Davydova; Curry, Stephen; Matthews, Stephen
Citation:

Citation: Simpson, Peter; Davydova, Natalia; Curry, Stephen; Matthews, Stephen. "Letter to the Editor: Resonance Assignment and Topology of the 2H, 13C, 15N labeled 29 kDa N-terminal Fragment of the Polypyrimidine Tract Binding Protein (PTB)"  J. Biomol. NMR 24, 79-80 (2002).

Assembly members:

Assembly members:
Human Polypyrimidine Tract Binding Protein, polymer, 263 residues, 28680 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology

Data sets:
Data typeCount
1H chemical shifts181
13C chemical shifts571
15N chemical shifts181

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1PTB1 domains 1 and 21

Entities:

Entity 1, PTB1 domains 1 and 2 263 residues - 28680 Da.

1   METARGGLYSERHISHISHISHISHISHIS
2   GLYSERSERARGSERSERGLYVALPROSER
3   ARGVALILEHISILEARGLYSLEUPROILE
4   ASPVALTHRGLUGLYGLUVALILESERLEU
5   GLYLEUPROPHEGLYLYSVALTHRASNLEU
6   LEUMETLEULYSGLYLYSASNGLNALAPHE
7   ILEGLUMETASNTHRGLUGLUALAALAASN
8   THRMETVALASNTYRTYRTHRSERVALTHR
9   PROVALLEUARGGLYGLNPROILETYRILE
10   GLNPHESERASNHISLYSGLULEULYSTHR
11   ASPSERSERPROASNGLNALAARGALAGLN
12   ALAALALEUGLNALAVALASNSERVALGLN
13   SERGLYASNLEUALALEUALAALASERALA
14   ALAALAVALASPALAGLYMETALAMETALA
15   GLYGLNSERPROVALLEUARGILEILEVAL
16   GLUASNLEUPHETYRPROVALTHRLEUASP
17   VALLEUHISGLNILEPHESERLYSPHEGLY
18   THRVALLEULYSILEILETHRPHETHRLYS
19   ASNASNGLNPHEGLNALALEULEUGLNTYR
20   ALAASPPROVALSERALAGLNHISALALYS
21   LEUSERLEUASPGLYGLNASNILETYRASN
22   ALACYSCYSTHRLEUARGILEASPPHESER
23   LYSLEUTHRSERLEUASNVALLYSTYRASN
24   ASNASPLYSSERARGASPTYRTHRARGPRO
25   ASPLEUPROSERGLYASPSERGLNPROSER
26   LEUASPGLNTHRMETALAALAALAPHEGLY
27   LEUSERVAL

Samples:

sample_1: Human Polypyrimidine Tract Binding Protein, [U-80% 2H; U-13C; U-15N], 0.5 mM; DTT 10 mM

Cond_1: pH*: 6.0; temperature: 298 K; ionic strength: 0.2 M

Experiments:

NameSampleSample stateSample conditions
1H-15N HSQCnot availablenot availablenot available
CT-HNCAnot availablenot availablenot available
2H-dec CT-HN(CO)CAnot availablenot availablenot available
2H-dec HNCOnot availablenot availablenot available
HN(CA)COnot availablenot availablenot available
2H-dec CT-HNCACBnot availablenot availablenot available
CBCA(CO)NHnot availablenot availablenot available

Software:

Aurelia v2.8.11 - Strip-based backbone assignment

NMR spectrometers:

  • Bruker DRX 500 MHz

Related Database Links:

BMRB 6177
PDB
DBJ BAC29560
GB EDL31647 EDL89385 EDL89387
REF NP_001269942 XP_008985119 XP_008985120 XP_010330760 XP_010805519
SP Q00438
AlphaFold Q00438

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks