BMRB Entry 5309

Title:
1H, 15N and 13C assignments of FLIN4, an intramolecular LMO4:ldb1 complex
Deposition date:
2002-03-04
Original release date:
2002-08-22
Authors:
Deane, Janet; Visvader, Jane; Mackay, Joel; Matthews, Jacqueline
Citation:

Citation: Deane, Janet; Visvader, Jane; Mackay, Joel; Matthews, Jacqueline. "Letter to the Editor: 1H, 15N and 13C assignments of FLIN4, an intramolecular LMO4:ldb1 complex"  J. Biomol. NMR 23, 165-166 (2002).
PubMed: 12153047

Assembly members:

Assembly members:
engineered intramolecular protein complex, polymer, 122 residues, 13004 Da.
ZN, non-polymer, 65.409 Da.

Natural source:

Natural source:   Common Name: mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculus

Experimental source:

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts393
15N chemical shifts116
1H chemical shifts693

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1flin41
2Zinc ion, I2
3Zinc ion, II2

Entities:

Entity 1, flin4 122 residues - 13004 Da.

1   GLYSERLEUSERTRPLYSARGCYSALAGLY
2   CYSGLYGLYLYSILEALAASPARGPHELEU
3   LEUTYRALAMETASPSERTYRTRPHISSER
4   ARGCYSLEULYSCYSSERSERCYSGLNALA
5   GLNLEUGLYASPILEGLYTHRSERSERTYR
6   THRLYSSERGLYMETILELEUCYSARGASN
7   ASPTYRILEARGLEUPHEGLYASNSERGLY
8   ALAGLYGLYSERGLYGLYHISMETGLYSER
9   GLYGLYASPVALMETVALVALGLYGLUPRO
10   THRLEUMETGLYGLYGLUPHEGLYASPGLU
11   ASPGLUARGLEUILETHRARGLEUGLUASN
12   THRGLNPHEASPALAALAASNGLYILEASP
13   ASPGLU

Entity 2, Zinc ion, I - Zn - 65.409 Da.

1   ZN

Samples:

sample_1: engineered intramolecular protein complex0.3 – 0.8 mM

sample_2: engineered intramolecular protein complex, [U-95% 15N], 0.3 – 0.8 mM

sample_3: engineered intramolecular protein complex, [U-95% 13C; U-95% 15N], 0.3 – 0.8 mM

cond_1: ionic strength: 0.22 M; pH: 7.0 na; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
1H TOCSYnot availablenot availablecond_1
1H NOESYnot availablenot availablecond_1
1H DQF-COSYnot availablenot availablecond_1
1H-15N HSQCnot availablenot availablecond_1
HNHAnot availablenot availablecond_1
HNCAnot availablenot availablecond_1
HN(CO)CAnot availablenot availablecond_1
HNCACBnot availablenot availablecond_1
CBCA(CO)NHnot availablenot availablecond_1
HNCOnot availablenot availablecond_1
HN(CA)COnot availablenot availablecond_1
CC(CO)NH-TOCSYnot availablenot availablecond_1
HCC(CO)NH-TOCSYnot availablenot availablecond_1
HCCH-TOCSYnot availablenot availablecond_1
15N-NOESY-HSQCnot availablenot availablecond_1

Software:

xwinnmr - data aquisition, processing

XEASY - assignments

NMR spectrometers:

  • BRUKER DMX 600 MHz

Related Database Links:

PDB

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks