BMRB Entry 5113

Title:
High-resolution Structures for the Psi-conotoxins Indicate Differences in Disulfide Bridge Dynamics
Deposition date:
2001-08-15
Original release date:
2008-07-17
Authors:
Van Wagoner, R.; Ireland, C.
Citation:

Citation: Van Wagoner, R.; Ireland, C.. "An Improved Solution Structure for Psi-conotoxin Piiie"  Biochemistry 42, 6347-6352 (2003).
PubMed: 12767215

Assembly members:

Assembly members:
psi-conotoxin, polymer, 24 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: purple cone   Taxonomy ID: 41690   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Conus purpurascens

Experimental source:

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
psi-conotoxin: HXXCCLYGKCRRYXGCSSAS CCQX

Data sets:
Data typeCount
1H chemical shifts136

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1psi-conotoxin PIIIE1

Entities:

Entity 1, psi-conotoxin PIIIE 24 residues - Formula weight is not available

1   HISHYPHYPCYSCYSLEUTYRGLYLYSCYS
2   ARGARGTYRHYPGLYCYSSERSERALASER
3   CYSCYSGLNAAR

Samples:

sample_1: psi-conotoxin, [U-13CB]-Cys4,Cys21, 16 mM; TFA 16 mM; H2O 90%; D2O 10%

sample_2: psi-conotoxin, [U-13CB]-Cys4,Cys21, 16 mM; D2O 100%

sample_cond_1: ionic strength: 16 mM; pH: 3.48; pressure: 1 atm; temperature: 277 K

sample_cond_2: ionic strength: 16 mM; pH: 3.48; pressure: 1 atm; temperature: 295 K

Experiments:

NameSampleSample stateSample conditions
3D 13C-separated NOESYnot availablenot availablenot available
DQF-COSYnot availablenot availablenot available
Half-filtered PE-COSYnot availablenot availablenot available
Double half-filtered 2D NOESYnot availablenot availablenot available

Software:

VNMR v6.1B - collection

FELIX v97.0 - processing

IRMA v97.0 - iterative matrix relaxation

DGII v97.0 - structure solution

DISCOVER v2.98 - structure solution

NMR spectrometers:

  • Varian UNITY 600 MHz

Related Database Links:

PDB