BMRB Entry 5067

Title:
Solution Structure of the Lipoyl Domain of the Chimeric Dihydrolipoyl Dehydrogenase P64K from Neisseria meningitidis
Deposition date:
2001-07-02
Original release date:
2001-11-14
Authors:
Tozawa, Kaeko; Broadhurst, Richard; Raine, Andrew; Fuller, Christopher; Alvarez, Anabel; Guillen, Gerardo; Padron, Gabriel; Perham, Richard
Citation:

Citation: Tozawa, Kaeko; Broadhurst, Richard; Raine, Andrew; Fuller, Christopher; Alvarez, Anabel; Guillen, Gerardo; Padron, Gabriel; Perham, Richard. "Solution Structure of the Lipoyl Domain of the Chimeric Dihydrolipoyl Dehydrogenase P64K from Neisseria meningitidis"  Eur. J. Biochem. 268, 4908-4917 (2001).
PubMed: 11559360

Assembly members:

Assembly members:
lipoyl domain, polymer, 81 residues, 8323 Da.

Natural source:

Natural source:   Common Name: Neisseria meningitidis   Taxonomy ID: 487   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Neisseria meningitidis

Experimental source:

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):

Data sets:
Data typeCount
1H chemical shifts575
13C chemical shifts265
15N chemical shifts80

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1p64K lipoyl domain1

Entities:

Entity 1, p64K lipoyl domain 81 residues - 8323 Da.

1   ALALEUVALGLULEULYSVALPROASPILE
2   GLYGLYHISGLUASNVALASPILEILEALA
3   VALGLUVALASNVALGLYASPTHRILEALA
4   VALASPASPTHRLEUILETHRLEUGLUTHR
5   ASPLYSALATHRMETASPVALPROALAGLU
6   VALALAGLYVALVALLYSGLUVALLYSVAL
7   LYSVALGLYASPLYSILESERGLUGLYGLY
8   LEUILEVALVALVALGLUALAGLUGLYTHR
9   ALA

Samples:

sample_1: lipoyl domain, [U-2H; U-13C; U-15N], 2 mM

condition_1: pH*: 5.5; temperature: 298 K; ionic strength: 20 mM; pressure: 1 atm

Experiments:

NameSampleSample stateSample conditions
1H-15N NOESYnot availablenot availablenot available
1H-15N TOCSYnot availablenot availablenot available
HNCAnot availablenot availablenot available
1H-13C TOCSYnot availablenot availablenot available
1H-13C NOESYnot availablenot availablenot available

Software:

ansig v3.3 - assignment

NMR spectrometers:

  • Bruker DRX 500 MHz

Related Database Links:

PDB
EMBL CAA54878 CAA57206 CAA59171 CAA61894 CAA61895
GB AAF41719 AAW89295 ABX73429 ACF30036 ADO31781
REF NP_274363 WP_002213261 WP_002217021 WP_002219145 WP_002220847

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks