BMRB Entry 4888

Name: Backbone 1H and 15N Chemical Shift Assignment of c-Src SH3 in high salt solution
Published: 2002-01-23

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR4888

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone 1H and 15N Chemical Shift Assignment of c-Src SH3 in high salt solution

Deposition date:

2000-10-30

Original release date:

2002-01-23

Authors:

Wang, Chunyu; Pawley, Norma; Nicholson, Linda

Citation:

Citation: Wang, Chunyu; Pawley, Norma; Nicholson, Linda. "The Role of Backbone Motions in Ligand Binding to the c-Src SH3 Domain" J. Mol. Biol. 313, 873-887 (2001).
PubMed: 11697910

Assembly members:

Assembly members:
Src Homology 3 domain, polymer, 72 residues, Formula weight is not available

Natural source:

Natural source: Common Name: chicken Taxonomy ID: 9031 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Gallus gallus

Experimental source:

Experimental source: Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Src Homology 3 domain: AGGVTTFVALYDYESRTETD LSFKKGERLQIVNNTEGDWW LAHSLTTGQTGYIPSNYVAP SDSIQAEGIHRD

Data sets:

assigned_chemical_shifts
- sh3_unlig

Data type	Count
15N chemical shifts	70
1H chemical shifts	70

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	c-Src SH3	1

Entities:

Entity 1, c-Src SH3 72 residues - Formula weight is not available

1

ALA

GLY

VAL

THR

PHE

VAL

ALA

LEU

2

TYR

ASP

TYR

GLU

SER

ARG

THR

GLU

THR

ASP

3

LEU

SER

PHE

LYS

GLY

GLU

ARG

LEU

GLN

4

ILE

VAL

ASN

THR

GLU

GLY

ASP

TRP

5

LEU

ALA

HIS

SER

LEU

THR

GLY

GLN

THR

6

GLY

TYR

ILE

PRO

SER

ASN

TYR

VAL

ALA

PRO

7

SER

ASP

SER

ILE

GLN

ALA

GLU

GLY

ILE

HIS

8

ARG

ASP

Samples:

sample_1: Src Homology 3 domain, [U-N15], 1 mM; sodium phosphate 50 mM; sodium sulphate 200 mM; DTT 10 mM; pepstatin 5 mg/ml; EDTA 50 uM; benzamidine 10 ug/ml; chloramphenicol 50 uM

condition_1: pH: 6.5; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
1H-15N NOESY	not available	not available	not available
1H-15N TOCSY	not available	not available	not available
HNCA	not available	not available	not available

Software:

NMRPipe -

NMR spectrometers:

Varian INOVA 600 MHz

BMRB	16750 3433
PDB	1FMK 1KSW 1NLO 1NLP 1PRL 1PRM 1QWE 1QWF 1RLP 1RLQ 1SRL 1SRM 1Y57 2H8H 2PTK 2SRC 3FJ5 4K11
DBJ	BAE26865 BAI47379
EMBL	CAA23696 CAA33404 CAA36154 CAA36156 CAA37004
GB	AAA42565 AAA42571 AAA42583 AAA49078 AAA60584
REF	NP_001020566 NP_001104274 NP_001248263 NP_005408 NP_056888
SP	P00523 P00524 P00525 P00526 P12931
TPG	DAA23281
AlphaFold	P00526 P00523 P00524 P00525 P12931