BMRB Entry 4876

Title:
1H,13C,15N assignment of Ca2+-bound state of Canine Milk Lysozyme at 30deg
Deposition date:
2000-10-24
Original release date:
2001-04-30
Authors:
Kobashigawa, Yoshihiro; Nitta, Katsutoshi; Tsuda, Sakae
Citation:

Citation: Kobashigawa, Yoshihiro; Miura, Kazunori; Demura, Makoto; Nemoto, Nobuaki; Koshiba, Takumi; Nitta, Katsutoshi; Tsuda, Sakae. "Letter to the Editor: Assignment of 1H, 13C, and 15N Resonances of Canine Milk Lysozyme"  J. Biomol. NMR 19, 387-388 (2001).

Assembly members:

Assembly members:
canine milk lysozyme, polymer, 130 residues, Formula weight is not available
CA, non-polymer, 40.078 Da.

Natural source:

Natural source:   Common Name: dog   Taxonomy ID: 9615   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Canis familiaris

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pet22(b)

Data sets:
Data typeCount
13C chemical shifts244
15N chemical shifts125
1H chemical shifts260

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1canine milk lysozyme1
2Ca2

Entities:

Entity 1, canine milk lysozyme 130 residues - Formula weight is not available

1   SERLYSILEPHESERLYSCYSGLULEUALA
2   ARGLYSLEULYSSERMETGLYMETASPGLY
3   PHEHISGLYTYRSERLEUALAASNTRPVAL
4   CYSMETALAGLUTYRGLUSERASNPHEASN
5   THRGLNALAPHEASNGLYARGASNSERASN
6   GLYSERSERASPTYRGLYILEPHEGLNLEU
7   ASNSERLYSTRPTRPCYSLYSSERASNSER
8   HISSERSERALAASNALACYSASNILEMET
9   CYSSERLYSPHELEUASPASPASNILEASP
10   ASPASPILEALACYSALALYSARGVALVAL
11   LYSASPPROASNGLYMETSERALATRPVAL
12   ALATRPVALLYSHISCYSLYSGLYLYSASP
13   LEUSERLYSTYRLEUALASERCYSASNLEU

Entity 2, Ca - Ca - 40.078 Da.

1   CA

Samples:

sample1: canine milk lysozyme, [U-15N; U-13C], 1.0 mM

Ex-cond_1: pH: 4.5; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
experiment_1sample1not availableEx-cond_1

Software:

No software information available

NMR spectrometers:

  • Varian Inova 500 MHz

Related Database Links:

BMRB 4883 4887
PDB
GenBank AAB31794
REF XP_851313
SWISS-PROT P81708

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks