BMRB Entry 4863

Title:
SOLUTION STRUCTURE OF NUCLEOLIN RBD2
Deposition date:
2000-10-17
Original release date:
2001-11-14
Authors:
Allain, F.; Gilbert, D.; Bouvet, P.; Feigon, J.
Citation:

Citation: Allain, F.; Gilbert, D.; Bouvet, P.; Feigon, J.. "Solution Strcuture of the two N-terminal RNA-binding Domains of Nucleolin and NMR Study of the Interaction with its RNA Target"  J. Mol. Biol. 303, 227-241 (2000).
PubMed: 11023788

Assembly members:

Assembly members:
NUCLEOLIN RBD2, polymer, 96 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: golden hamster   Taxonomy ID: 10036   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mesocricetus auratus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: BL21(DE3)

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts252
15N chemical shifts95
1H chemical shifts589

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1NUCLEOLIN RBD21

Entities:

Entity 1, NUCLEOLIN RBD2 96 residues - Formula weight is not available

1   SERHISMETLEUGLUASPPROCYSTHRSER
2   LYSLYSVALARGALAALAARGTHRLEULEU
3   ALALYSASNLEUSERPHEASNILETHRGLU
4   ASPGLULEULYSGLUVALPHEGLUASPALA
5   LEUGLUILEARGLEUVALSERGLNASPGLY
6   LYSSERLYSGLYILEALATYRILEGLUPHE
7   LYSSERGLUALAASPALAGLULYSASNLEU
8   GLUGLULYSGLNGLYALAGLUILEASPGLY
9   ARGSERVALSERLEUTYRTYRTHRGLYGLU
10   LYSGLYGLYTHRARGGLY

Samples:

sample_1: NUCLEOLIN RBD2, [U-15N; U-13C], 1 mM; potassium phosphate 50 mM; KCl 100 mM; DTT, [U-2H], 5 mM; H2O 90%; D2O 10%

sample_2: NUCLEOLIN RBD2, [U-15N; U-13C], 1 mM; potassium phosphate 50 mM; KCl 100 mM; DTT, [U-2H], 5 mM; D2O 100%

sample_cond_1: ionic strength: 150 mM; pH: 6.2; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
3D 13C-separated NOESYnot availablenot availablenot available
3D 15N-separated NOESYnot availablenot availablenot available
2D NOESYnot availablenot availablenot available

Software:

X-PLOR v3.841 - refinement, structure solution

DYANA v1 - structure solution

NMR spectrometers:

  • Bruker DRX 500 MHz

Related Database Links:

PDB
GB EGW00584

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks