BMRB Entry 4610

Title:
NMR Observation of A-tetrad in a DNA Quadruplex
Deposition date:
2000-05-31
Original release date:
2000-12-22
Authors:
Patel, P.; Koti, A.; Hosur, R.
Citation:

Citation: Patel, P.; Koti, A.; Hosur, R.. "NMR studies on truncated sequences of human telomeric DNA: observation of a novel A-tetrad"  Nucleic Acids Res. 27, 3836-3843 (1999).

Assembly members:

Assembly members:
AGGGT, polymer, 5 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
AGGGT: AGGGT

Data sets:
Data typeCount
1H chemical shifts29

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
15'-D(*AP*GP*GP*GP*T)-3', strand 1a1
25'-D(*AP*GP*GP*GP*T)-3', strand 1b1
35'-D(*AP*GP*GP*GP*T)-3', strand 2a1
45'-D(*AP*GP*GP*GP*T)-3', strand 2b1

Entities:

Entity 1, 5'-D(*AP*GP*GP*GP*T)-3', strand 1a 5 residues - Formula weight is not available

1   DADGDGDGDT

Samples:

sample_1: AGGGT1 – 2 mM; potassium phosphate 2 mM; EDTA 0.2 mM; KCl 100.0 mM; H2O 90%; D2O 10%

sample_2: AGGGT1 – 2 mM; potassium phosphate 2 mM; EDTA 0.2 mM; KCl 100.0 mM; D2O 100%

sample_cond_1: pH: 7.3; pressure: 1 atm

Experiments:

NameSampleSample stateSample conditions
2D NOESYnot availablenot availablesample_cond_1
2D TOCSYnot availablenot availablesample_cond_1
E-COSYnot availablenot availablesample_cond_1

Software:

VNMR v6.1 - data collection

FELIX v230 and 97 - data analysis

xwinnmr v1.3 - data collection

DISCOVER v3.1 - structure solution

IRMA v2.3 - iterative matrix relaxation

NMR spectrometers:

  • Bruker AMX 500 MHz