BMRB Entry 4571

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4571

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Title:
Zinc-bundle Structure of the Essential RNA Polymerase Subunit RPB10 from Methanobacterium thermoautotrophicum
Deposition date:
2000-01-13
Original release date:
2000-12-19
Authors:
Mackereth, Cameron; Arrowsmith, Cheryl; Edwards, Aled; McIntosh, Lawrence
Citation:

Citation: Mackereth, Cameron; Arrowsmith, Cheryl; Edwards, Aled; McIntosh, Lawrence. "Zinc-bundle Structure of the Essential RNA Polymerase Subunit RPB10 from Methanobacteriumthermoautotrophicum"  Proc. Natl. Acad. Sci. U. S. A. 97, 6316-6321 (2000).

Assembly members:

Assembly members:
RNA polymerase subunit RPB10, polymer, 58 residues, 8464 Da.
ZN, non-polymer, 65.409 Da.

Natural source:

Natural source:   Common Name: 2166   Taxonomy ID: 187420   Superkingdom: archaea   Kingdom: not available   Genus/species: Methanobacterium thermoautotrophicum

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET15b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
RNA polymerase subunit RPB10: GSHMIPVRCLSCGKPVSAYF NEYQRRVADGEDPKDVLDDL GLKRYCCRRMLISHVETW

Data sets:
  • assigned_chemical_shifts
Data typeCount
1H chemical shifts384
13C chemical shifts243
15N chemical shifts54

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1RPB101
2Zn(II)2

Entities:

Entity 1, RPB10 58 residues - 8464 Da.

1   GLYSERHISMETILEPROVALARGCYSLEU
2   SERCYSGLYLYSPROVALSERALATYRPHE
3   ASNGLUTYRGLNARGARGVALALAASPGLY
4   GLUASPPROLYSASPVALLEUASPASPLEU
5   GLYLEULYSARGTYRCYSCYSARGARGMET
6   LEUILESERHISVALGLUTHRTRP

Entity 2, Zn(II) - Zn - 65.409 Da.

1   ZN

Samples:

N15-labelled: RNA polymerase subunit RPB10, [U-100% 15N], 0.5 mM

C13_N15-labelled: RNA polymerase subunit RPB10, [U-100% 13C; U-100% 15N], 0.5 mM

10%_C13-labelled: RNA polymerase subunit RPB10, [U-10% 13C], 0.5 mM

H2O: pH: 7.5 na; temperature: 303 K

D2O: pH*: 7.1 na; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
not availablenot availablenot availablenot available

Software:

FELIX v95, Biosym Technologies -

NMR spectrometers:

  • Varian Unity 500 MHz

Related Database Links:

PDB
GB AAB84548 ADL58125
REF WP_010875681 WP_013295349
SP O26147
AlphaFold O26147

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks