BMRB Entry 4307

Title:
1H, 13C, and 15N Chemical Shift Assignments for SHa rPrP(90-231).
Deposition date:
1999-02-16
Original release date:
2000-05-10
Authors:
James, Thomas; Liu, He; Ulyanov, Nikolai; Far-Jones, Shauna
Citation:

Citation: Liu, H.; Farr-Jones, S.; Ulyanov, N.; Llinas, M.; Marqusee, S.; Groth, D.; Cohen, F.; Prusiner, S.; James, T.. "Solution structure of Syrian hamster prion protein rPrP(90-231)"  Biochemistry 38, 5362-5377 (1999).
PubMed: 10220323

Assembly members:

Assembly members:
recombinant Syrian hamster prion protein, polymer, 142 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Syrian hamster   Taxonomy ID: 10036   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Microcricetus aureus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Data sets:
Data typeCount
13C chemical shifts401
15N chemical shifts168
1H chemical shifts862

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1rPrP(90-231)1

Entities:

Entity 1, rPrP(90-231) 142 residues - Formula weight is not available

1   GLYGLNGLYGLYGLYTHRHISASNGLNTRP
2   ASNLYSPROSERLYSPROLYSTHRASNMET
3   LYSHISMETALAGLYALAALAALAALAGLY
4   ALAVALVALGLYGLYLEUGLYGLYTYRMET
5   LEUGLYSERALAMETSERARGPROMETMET
6   HISPHEGLYASNASPTRPGLUASPARGTYR
7   TYRARGGLUASNMETASNARGTYRPROASN
8   GLNVALTYRTYRARGPROVALASPGLNTYR
9   ASNASNGLNASNASNPHEVALHISASPCYS
10   VALASNILETHRILELYSGLNHISTHRVAL
11   THRTHRTHRTHRLYSGLYGLUASNPHETHR
12   GLUTHRASPILELYSILEMETGLUARGVAL
13   VALGLUGLNMETCYSTHRTHRGLNTYRGLN
14   LYSGLUSERGLNALATYRTYRASPGLYARG
15   ARGSER

Samples:

sample_1: recombinant Syrian hamster prion protein, [U-13C; U-15N], 0.7 – 1.3 mM

cond._set_1: pH: 5.2; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
HNCAnot availablenot availablenot available
HNCACBnot availablenot availablenot available
CBCA(CO)NHnot availablenot availablenot available
HNCOnot availablenot availablenot available
HBHA(CO)NHnot availablenot availablenot available
C(CO)NH-TOCSYnot availablenot availablenot available
HCCH-TOCSYnot availablenot availablenot available
1H-15N NOESY-HSQCnot availablenot availablenot available

Software:

SPARKY v3.6 - anotation, assignment, bookkeeping, peak picking

NMR spectrometers:

  • Bruker AMX 500 MHz
  • Bruker DRX 600 MHz
  • Bruker RMX 750 MHz
  • Varian Unity Plus 600 MHz

Related Database Links:

BMRB 17034 17834
PDB
GB AAA37090 AAA37091 AAA37092 AAA37093 AAB25731
REF XP_005068717 XP_012967855
SP P04273
AlphaFold P04273

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks