BMRB Entry 4290

Title:
Sequence-specific 1H Assignment of Antimicrobial Protein Tachycitin.
Deposition date:
1999-01-04
Original release date:
2000-10-30
Authors:
Suetake, Tetsuya; Tsuda, Sakae; Kawabata, Shun-ichiro; Kawano, Keiichi; Miura, Kazunori; Iwanaga, Sadaaki; Hikichi, Kunio; Nitta, Katsutoshi; Keiichi, Kawano
Citation:

Citation: Suetake, Tetsuya; Tsuda, Sakae; Kawabata, Shun-ichiro; Kawano, Keiichi; Miura, Kazunori; Iwanaga, Sadaaki; Hikichi, Kunio; Nitta, Katsutoshi; Keiichi, Kawano. "Chitin-binding Proteins in Invertebrates and Plants Comprise a Common Chitin-binding Structural Motif"  J. Biol. Chem. 275, 17929-17932 (2000).
PubMed: 10770921

Assembly members:

Assembly members:
tachycitin, polymer, 73 residues, 8364 Da.

Natural source:

Natural source:   Common Name: Japanese horseshoe crab   Taxonomy ID: 6853   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Tachypleus tridentatus

Experimental source:

Experimental source:   Production method: purified from the natural source

Entity Sequences (FASTA):

Data sets:
Data typeCount
1H chemical shifts496

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1tachycitin1

Entities:

Entity 1, tachycitin 73 residues - 8364 Da.

1   TYRLEUALAPHEARGCYSGLYARGTYRSER
2   PROCYSLEUASPASPGLYPROASNVALASN
3   LEUTYRSERCYSCYSSERPHETYRASNCYS
4   HISLYSCYSLEUALAARGLEUGLUASNCYS
5   PROLYSGLYLEUHISTYRASNALATYRLEU
6   LYSVALCYSASPTRPPROSERLYSALAGLY
7   CYSTHRSERVALASNLYSGLUCYSHISLEU
8   TRPLYSTHR

Samples:

sample_one: tachycitin1.0 – 2.0 mM

sample_conditions_one: pH: 4.2; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H DQF-COSYnot availablenot availablenot available
2D 1H-1H TOCSYnot availablenot availablenot available
2D 1H-1H NOESYnot availablenot availablenot available

Software:

Pipp v4.0.4 - peak picking

NMR spectrometers:

  • JEOL Alpha 600 MHz
  • JEOL Alpha 500 MHz

Related Database Links:

PDB
DBJ BAA12864