BMRB Entry 4188

Title:
C2 Domain of Cytosolic Phospholipase A2
Deposition date:
1998-08-18
Original release date:
2000-03-08
Authors:
Xu, Guang-Yi; McDonagh, Thomas; Yu, Hsiang-Ai; Nalefski, Eric; Clark, James; Cumming, Dale
Citation:

Citation: Xu, Guang-Yi; McDonagh, Thomas; Yu, Hsiang-Ai; Nalefski, Eric; Clark, James; Cumming, Dale. "Solution Structure and Membrane Interactions of the C2 Domain of Cytosolic Phospholipase A2"  J. Mol. Biol. 280, 485-500 (1998).

Assembly members:

Assembly members:
C2 Domain of Cytosolic Phospholipase A2, polymer, 138 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eucaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pTrcHisB(Invitrogen)

Data sets:
Data typeCount
13C chemical shifts576
15N chemical shifts140
1H chemical shifts969
coupling constants99

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1cPLA2-C21

Entities:

Entity 1, cPLA2-C2 138 residues - Formula weight is not available

1   METSERPHEILEASPPROTYRGLNHISILE
2   ILEVALGLUHISGLNTYRSERHISLYSPHE
3   THRVALVALVALLEUARGALATHRLYSVAL
4   THRLYSGLYALAPHEGLYASPMETLEUASP
5   THRPROASPPROTYRVALGLULEUPHEILE
6   SERTHRTHRPROASPSERARGLYSARGTHR
7   ARGHISPHEASNASNASPILEASNPROVAL
8   TRPASNGLUTHRPHEGLUPHEILELEUASP
9   PROASNGLNGLUASNVALLEUGLUILETHR
10   LEUMETASPALAASNTYRVALMETASPGLU
11   THRLEUGLYTHRALATHRPHETHRVALSER
12   SERMETLYSVALGLYGLULYSLYSGLUVAL
13   PROPHEILEPHEASNGLNVALTHRGLUMET
14   VALLEUGLUMETSERLEUGLUVAL

Samples:

sample_one: C2 Domain of Cytosolic Phospholipase A2, [U-95% 13C; U-95% 15N], 0.5 mM

sample_conditions_one: ionic strength: 0.0 M; pH*: 7.1; pressure: 1 atm; temperature: 308 K

Experiments:

NameSampleSample stateSample conditions
NOESYsample_onenot availablenot available
TOCSYsample_onenot availablenot available

Software:

No software information available

NMR spectrometers:

  • Varian Unity Plus 600 MHz

Related Database Links:

PDB
DBJ BAE26047 BAF83025 BAG64096 BAI46198
EMBL CAH92005
GB AAA60105 AAB00789 AAB00796 AAB33847 AAC21591
PRF 2022317A
REF NP_001075312 NP_001126164 NP_001298122 NP_032895 NP_077734
SP O77793 P47712 P47713 P50393 Q5R8A5
AlphaFold O77793 P47712 P47713 P50393 Q5R8A5

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks