BMRB Entry 4183

Title:
Expression and Assignment of 1H, 15N,and 13C Resonances of the C-terminal Domain of the Diphtheria Toxin Repressor
Deposition date:
1998-08-13
Original release date:
1999-03-01
Authors:
Twigg, Pamela; Wylie, Gregory; Wang, Guangshun; Caspar, Donald; Murphy, John; Logan, Timothy
Citation:

Citation: Twigg, Pamela; Wylie, Gregory; Wang, Guangshun; Caspar, Donald; Murphy, John; Logan, Timothy. "Expression and Assignment of 1H, 15N,and 13C Resonances of the C-terminal Domain of the Diphtheria Toxin Repressor"  J. Biomol. NMR 13, 197-198 (1999).

Assembly members:

Assembly members:
DtxR subunit 3, C-terminal domain, polymer, 109 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Corynebacterium diphtheriae   Taxonomy ID: 1717   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Corynebacterium diphtheriae

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pQE31

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts392
15N chemical shifts106
1H chemical shifts619

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1DtxR(130-226)1

Entities:

Entity 1, DtxR(130-226) 109 residues - Formula weight is not available

1   METARGGLYSERHISHISHISHISHISHIS
2   GLYSERASNPROILEPROGLYLEUASPGLU
3   LEUGLYVALGLYASNSERASPALAALAALA
4   PROGLYTHRARGVALILEASPALAALATHR
5   SERMETPROARGLYSVALARGILEVALGLN
6   ILEASNGLUILEPHEGLNVALGLUTHRASP
7   GLNPHETHRGLNLEULEUASPALAASPILE
8   ARGVALGLYSERGLUVALGLUILEVALASP
9   ARGASPGLYHISILETHRLEUSERHISASN
10   GLYLYSASPVALGLULEULEUASPASPLEU
11   ALAHISTHRILEARGILEGLUGLULEU

Samples:

sample_one: DtxR subunit 3, C-terminal domain, [U-95% 15N], 1 – 2 mM

sample_two: DtxR subunit 3, C-terminal domain, [U-95% 15N; U-95% 13C], 1 – 2 mM

sample_conditions_one: ionic strength: 0.2 na; pH: 6.5 na; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions

Software:

No software information available

NMR spectrometers:

  • unknown unknown 0 MHz

Related Database Links:

BMRB 15254 15255
PDB
EMBL CAE49945 CCG27916 CCG27917 CCG27918 CCG27919
GB AAA23296 AAA23299 AAA23301 AAU93781 AAU93782
REF WP_003851803 WP_010935052 WP_014316797 WP_014320173
SP H2I233 P0DJL7
AlphaFold H2I233 P0DJL7

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks