BMRB Entry 36114

Title:
NMR structure of YAP1-2 WW1 domain with LATS1 PPxY motif complex
Deposition date:
2017-09-15
Original release date:
2019-08-21
Authors:
Fan, J.; Sivaraman, J.
Citation:

Citation: Verma, Apoorva; Jing-Song, Fan; Finch-Edmondson, Megan; Velazquez-Campoy, Adrian; Balasegaran, Shanker; Sudol, Marius; Sivaraman, Jayaraman. "Biophysical studies and NMR structure of YAP2 WW domain - LATS1 PPxY motif complexes reveal the basis of their interaction"  Oncotarget 9, 8068-8080 (2018).
PubMed: 29487715

Assembly members:

Assembly members:
entity_1, polymer, 59 residues, 6578.255 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Cloning vector pGEM3_Zf

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts170
15N chemical shifts56
1H chemical shifts356

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1entity_11

Entities:

Entity 1, entity_1 59 residues - 6578.255 Da.

1   SERPHEGLUILEPROASPASPVALPROLEU
2   PROALAGLYTRPGLUMETALALYSTHRSER
3   SERGLYGLNARGTYRPHELEUASNHISILE
4   ASPGLNTHRTHRTHRTRPGLNASPPROARG
5   LYSALAMETGLYGLYGLYGLYASNTYRGLN
6   GLYPROPROPROPROTYRPROLYSHIS

Samples:

sample_1: WW domain with PPxY motif, [U-100% 13C; U-100% 15N], 1.0 ± 0.1 mM; H2O 90%; D2O, [U-2H], 10%

sample_2: WW domain with PPxY motif 1 ± 0.1 mM; D2O, [U-2H], 100%

sample_conditions_1: ionic strength: 100 mM; pH: 6.0; pressure: 1 atm; temperature: 298 K

sample_conditions_2: ionic strength: 100 mM; pH: 6.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H NOESYsample_2isotropicsample_conditions_2
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_1isotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

SPARKY, Goddard - data analysis

X-PLOR NIH, Schwieters, Kuszewski, Tjandra and Clore - structure calculation

NMR spectrometers:

  • Bruker Avance 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks