BMRB Entry 36027

Title:
Solution structure of human Gelsolin protein domain 1 at pH 7.3
Deposition date:
2016-10-26
Original release date:
2017-10-30
Authors:
Fan, J.; Yang, D.
Citation:

Citation: Fan, Jing-Song; Goh, Honzhen; Ding, Ke; Xue, Bo; Robinson, Robert; Yang, Daiwen. "Structural Basis for pH-mediated Regulation of F-actin Severing by Gelsolin Domain 1."  Sci. Rep. 7, 45230-45230 (2017).
PubMed: 28349924

Assembly members:

Assembly members:
entity_1, polymer, 133 residues, 14973.815 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Data sets:
Data typeCount
13C chemical shifts377
15N chemical shifts126
1H chemical shifts704

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1entity_11

Entities:

Entity 1, entity_1 133 residues - 14973.815 Da.

1   GLUHISPROGLUPHELEULYSALAGLYLYS
2   GLUPROGLYLEUGLNILETRPARGVALGLU
3   LYSPHEASPLEUVALPROVALPROTHRASN
4   LEUTYRGLYASPPHEPHETHRGLYASPALA
5   TYRVALILELEULYSTHRVALGLNLEUARG
6   ASNGLYASNLEUGLNTYRASPLEUHISTYR
7   TRPLEUGLYASNGLUCYSSERGLNASPGLU
8   SERGLYALAALAALAILEPHETHRVALGLN
9   LEUASPASPTYRLEUASNGLYARGALAVAL
10   GLNHISARGGLUVALGLNGLYPHEGLUSER
11   ALATHRPHELEUGLYTYRPHELYSSERGLY
12   LEULYSTYRLYSLYSGLYGLYVALALASER
13   GLYPHELYSHISVALVALPROASNGLUVAL
14   VALVALGLN

Samples:

sample_1: human Gelsolin, [U-13C; U-15N], 0.70 ± 0.1 mM; H2O 90%; D2O, [U-2H], 10%

sample_conditions_1: ionic strength: 0 M; pH: 7.3; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
4D NOESYsample_1isotropicsample_conditions_1

Software:

xplopr-nih v2.42, C.D. Schwieters, J.J. Kuszewski, N. Tjandra, and G.M. Clore - structure calculation

xplor-nih v2.42, C.D. Schwieters, J.J. Kuszewski, N. Tjandra, and G.M. Clore - refinement

SPARKY v3.13, Goddard - chemical shift assignment, peak picking

NMR spectrometers:

  • Bruker Avance 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks