BMRB Entry 30656

Title:
Solution NMR structure of the DNAJB6b deltaST variant (Aligned on the CTD domain)
Deposition date:
2019-08-22
Original release date:
2019-10-04
Authors:
Karamanos, T.; Clore, G.
Citation:

Citation: Karamanos, T.; Tugarinov, V.; Clore, G.. "Unraveling the structure and dynamics of the human DNAJB6b chaperone by NMR reveals insights into Hsp40-mediated proteostasis"  Proc. Natl. Acad. Sci. U. S. A. 116, 21529-21538 (2019).
PubMed: 31591220

Assembly members:

Assembly members:
entity_1, polymer, 190 residues, 21831.303 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli BL21(DE3)   Vector: pET15b

Data sets:
Data typeCount
13C chemical shifts535
15N chemical shifts159
1H chemical shifts283

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1entity_11

Entities:

Entity 1, entity_1 190 residues - 21831.303 Da.

1   GLYMETVALASPTYRTYRGLUVALLEUGLY
2   VALGLNARGHISALASERPROGLUASPILE
3   LYSLYSALATYRARGLYSLEUALALEULYS
4   TRPHISPROASPLYSASNPROGLUASNLYS
5   GLUGLUALAGLUARGLYSPHELYSGLNVAL
6   ALAGLUALATYRGLUVALLEUSERASPALA
7   LYSLYSARGASPILETYRASPLYSTYRGLY
8   LYSGLUGLYLEUASNGLYGLYGLYGLYGLY
9   GLYSERHISPHEASPSERPROPHEGLUPHE
10   GLYPHETHRPHEARGASNPROASPASPVAL
11   PHEARGGLUPHEPHEGLYGLYARGASPPRO
12   PHESERPHEASPPHEPHEGLUASPPROPHE
13   GLUASPPHEPHEGLYASNARGARGGLYPRO
14   ARGGLYGLYMETGLYASNPHELYSSERILE
15   SERTHRSERTHRLYSMETVALASNGLYARG
16   LYSILETHRTHRLYSARGILEVALGLUASN
17   GLYGLNGLUARGVALGLUVALGLUGLUASP
18   GLYGLNLEULYSSERLEUTHRILEASNGLY
19   LYSGLUGLNLEULEUARGLEUASPASNLYS

Samples:

sample_1: DNAJB6b deltaST variant, [U-13C; U-15N; U-2H], 2e-04 M; sodium chloride 0.05 M; sodium azide 0.02 % w/v; sodium phosphate 0.02 M

sample_2: DNAJB6b deltaST variant, [ILV-CH3; U-13C; U-15N; U-2H], 2e-04 M; sodium chloride 0.05 M; sodium azide 0.02 % w/v; sodium phosphate 0.02 M

sample_3: DNAJB6b deltaST variant, [ILV-CH3; U-15N; U-2H], 2e-04 M; sodium chloride 0.05 M; sodium azide 0.02 % w/v; sodium phosphate 0.02 M

sample_4: DNAJB6b deltaST variant, [U-13C; U-15N], 2e-04 M; sodium chloride 0.05 M; sodium azide 0.02 % w/v; sodium phosphate 0.02 M

sample_5: DNAJB6b deltaST variant, [U-15N;U-2H], 2e-04 M; sodium chloride 0.05 M; sodium azide 0.02 % w/v; sodium phosphate 0.02 M

sample_6: DNAJB6b deltaST variant, [U-15N;U-2H], 2e-04 M; sodium chloride 0.1 M; sodium azide 0.02 % w/v; sodium phosphate 0.02 M

sample_7: DNAJB6b deltaST variant, [U-15N;U-2H], 0.0002 M; sodium chloride 0.05 M; sodium azide 0.02 % w/v; sodium phosphate 0.02 M

sample_conditions_1: ionic strength: 87 mM; pH: 6.7; pressure: 1 atm; temperature: 298 K

sample_conditions_2: ionic strength: 145 mM; pH: 7; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N TROSYsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
2D 1H-13C HMQCsample_3isotropicsample_conditions_1
2D 1H-13C HMQC constant timesample_2isotropicsample_conditions_1
3D HMCMCBCAsample_2isotropicsample_conditions_1
3D HMCM(CBCA)COsample_2isotropicsample_conditions_1
3D HHN NOESY-TROSYsample_5isotropicsample_conditions_1
3D HHC NOESY-HMQCsample_3isotropicsample_conditions_1
3D HHC HMQC-NOESYsample_3isotropicsample_conditions_1
3D HCC HMQC-NOESY-HMQCsample_3isotropicsample_conditions_1
2D HN PREsample_5isotropicsample_conditions_1
2D HN ARTSY PF1sample_6anisotropicsample_conditions_2
3D HNCOsample_4isotropicsample_conditions_1
2D HN ARTSY PegHexsample_7anisotropicsample_conditions_1

Software:

CS-ROSETTA, Shen, Vernon, Baker and Bax - refinement, structure calculation

CcpNmr Analysis, CCPN - chemical shift assignment

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMRDraw, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - peak picking

X-PLOR NIH, Schwieters, Kuszewski, Tjandra and Clore - structure calculation

NMR spectrometers:

  • Bruker AVANCE III 500 MHz
  • Bruker AVANCE III 600 MHz
  • Bruker AVANCE III 800 MHz
  • Bruker AVANCE III 900 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks