BMRB Entry 30311

Title:
Solution structure of phage displayed derived peptide inhibitor of frizzled 7 receptor
Deposition date:
2017-06-22
Original release date:
2018-04-11
Authors:
Fu, Y.
Citation:

Citation: Nile, Aaron; de Sousa E Melo, Felipe; Mukund, Susmith; Piskol, Robert; Hansen, Simon; Zhou, Lijuan; Zhang, Yingnan; Fu, Yue; Gogol, Emily; Komuves, Laszlo; Modrusan, Zora; Angers, Stephane; Franke, Yvonne; Koth, Christopher; Fairbrother, Wayne; Wang, Weiru; de Sauvage, Frederic; Hannoush, Rami. "A selective peptide inhibitor of Frizzled 7 receptors disrupts intestinal stem cells"  Nat. Chem. Biol. 14, 582-590 (2018).
PubMed: 29632413

Assembly members:

Assembly members:
Fz7 binding peptide, polymer, 14 residues, 1755.986 Da.

Natural source:

Natural source:   Common Name: not available   Taxonomy ID: 32644   Superkingdom: not available   Kingdom: not available   Genus/species: not available not available

Experimental source:

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Fz7 binding peptide: LPSDDLEFWCHVMY

Data sets:
Data typeCount
1H chemical shifts178

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1entity_1, chain A1
2entity_1, chain B1

Entities:

Entity 1, entity_1, chain A 14 residues - 1755.986 Da.

1   LEUPROSERASPASPLEUGLUPHETRPCYS
2   HISVALMETTYR

Samples:

sample_1: Fz binding peptide 1 mM; acentonitrile 10%; sodium phosphate 10 mM

sample_conditions_1: ionic strength: 10 mM; pH: 7.3; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1

Software:

Analysis, CCPN - chemical shift assignment, data analysis, peak picking

CNS, Brunger, Adams, Clore, Gros, Nilges and Read - refinement

CYANA, Guntert, Mumenthaler and Wuthrich - structure calculation

NMR spectrometers:

  • Bruker ultrashield plus 600 MHz