BMRB Entry 30289

Title:
Solution NMR Structure of the C-terminal Headpiece Domain of Villin 4 from A.thaliana, the First Non-Vertebrate Headpiece Structure
Deposition date:
2017-05-01
Original release date:
2017-05-08
Authors:
Miears, H.; Smirnov, S.
Citation:

Citation: Miears, Heather; Gruber, David; Horvath, Nicholas; Antos, John; Young, Jeff; Sigurjonsson, Johann; Klem, Maya; Rosenkranz, Erin; Okon, Mark; McKnight, C James; Vugmeyster, Liliya; Smirnov, Serge. "Plant Villin Headpiece Domain Demonstrates a Novel Surface Charge Pattern and High Affinity for F-Actin"  Biochemistry 57, 1690-1701 (2018).
PubMed: 29444403

Assembly members:

Assembly members:
entity_1, polymer, 63 residues, 7500.623 Da.

Natural source:

Natural source:   Common Name: Mouse-ear cress   Taxonomy ID: 3702   Superkingdom: Eukaryota   Kingdom: Viridiplantae   Genus/species: Arabidopsis thaliana

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts253
15N chemical shifts66
1H chemical shifts320

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1entity_11

Entities:

Entity 1, entity_1 63 residues - 7500.623 Da.

1   LEUPROALAHISPROTYRASPARGLEULYS
2   THRTHRSERTHRASPPROVALSERASPILE
3   ASPVALTHRARGARGGLUALATYRLEUSER
4   SERGLUGLUPHELYSGLULYSPHEGLYMET
5   THRLYSGLUALAPHETYRLYSLEUPROLYS
6   TRPLYSGLNASNLYSPHELYSMETALAVAL
7   GLNLEUPHE

Samples:

sample_1: PIPES, none, 20.0 mM; atVHP, none, 1.0 mM; sodium azide, none, 0.01%; sodium chloride, none, 50 mM

sample_2: PIPES, none, 20.0 mM; atVHP, none, 1.0 mM; sodium azide, none, 0.01%; sodium chloride, none, 50 mM

sample_conditions_1: ionic strength: 50 mM; pH: 6.8; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
15N-HSQCsample_1isotropicsample_conditions_1
13C-HSQCsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESY aliphaticsample_1isotropicsample_conditions_1
3D 1H-13C NOESY aromaticsample_1isotropicsample_conditions_1
3D C(CO)NHsample_1isotropicsample_conditions_1
3D HCC(CO)NHsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1
2D HBCBCGCDHDsample_1isotropicsample_conditions_1

Software:

CYANA, Guntert, Mumenthaler and Wuthrich - refinement

CYANA v2.1, Guntert, Mumenthaler and Wuthrich - structure calculation

NMRPipe v8.9, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMRView v9.0, Johnson, One Moon Scientific - peak picking

TOPSPIN v3.1, Bruker Biospin - collection

NMR spectrometers:

  • Bruker AvanceIII 500 MHz
  • Bruker AvanceIII 600 MHz
  • Bruker AvanceIII 850 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks