BMRB Entry 30079

Title:
NMR structure of Uncharacterized protein from Pseudomonas aeruginosa PAO1
Deposition date:
2016-05-09
Original release date:
2016-06-09
Authors:
Barnwal, R.; Varani, G.; Seattle Structural Genomics Center for Infectious Disease (SSGCID), SSGCID
Citation:

Citation: Barnwal, R.; Varani, G.. "NMR structure of Uncharacterized protein from Pseudomonas aeruginosa PAO1"  .

Assembly members:

Assembly members:
Uncharacterized protein, polymer, 94 residues, 10693.325 Da.

Natural source:

Natural source:   Common Name: g-proteobacteria   Taxonomy ID: 208964   Superkingdom: Bacteria   Kingdom: not available   Genus/species: not available not available

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):

Data typeCount
13C chemical shifts413
15N chemical shifts91
1H chemical shifts622

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1entity_11

Entities:

Entity 1, entity_1 94 residues - 10693.325 Da.

1   METTHRPROILEGLUTYRILEASPARGALA
2   LEUALALEUVALVALASPARGLEUALAARG
3   TYRPROGLYTYRGLUVALLEULEUSERALA
4   GLULYSGLNLEUGLNTYRILEARGSERVAL
5   LEULEUASPARGSERLEUASPARGSERALA
6   LEUHISARGLEUTHRLEUGLYSERILEALA
7   VALLYSGLUPHEASPGLUTHRASPPROGLU
8   LEUSERARGALALEULYSASPALATYRTYR
9   VALGLYILEARGTHRGLYARGGLYLEULYS
10   VALASPLEUPRO

Samples:

sample_1: Uncharacterized protein, [U-98% 13C; U-98% 15N], 1.1 mM; H2O 95%; D2O 5%; NaCl 100 mM; MES 20 mM

sample_conditions_1: ionic strength: 100 mM; pH: 6.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D H(CCO)NHsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1

Software:

CcpNMR, CCPN - chemical shift assignment, peak picking

TOPSPIN, Bruker Biospin - collection, processing

CYANA v3.97, GUNTERT, MUMENTHALER AND WUTHRICH - refinement, structure calculation

NMR spectrometers:

  • Bruker AvanceII 600 MHz
  • Bruker AvanceIII 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks