BMRB Entry 26593

Title:
Human Obscurin Ig59
Deposition date:
2015-06-25
Original release date:
2016-07-11
Authors:
Caldwell, Tracy; Wright, Nathan
Citation:

Citation: Caldwell, Tracy; Wright, Nathan. "Solution Structure of Ig59"  .

Assembly members:

Assembly members:
Obscurin_Ig59, polymer, 104 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pet24A

Data sets:
Data typeCount
13C chemical shifts356
15N chemical shifts94
1H chemical shifts556

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Obscurin Ig591

Entities:

Entity 1, Obscurin Ig59 104 residues - Formula weight is not available

Residues 99-104 represent a non-native His-tag

1   TRPARGLEUGLUILELEUGLULEULEULYS
2   ASNALAALAVALARGALAGLYALAGLNALA
3   ARGPHETHRCYSTHRLEUSERGLUALAVAL
4   PROVALGLYGLUALASERTRPTYRILEASN
5   GLYALAALAVALGLNPROASPASPSERASP
6   TRPTHRVALTHRALAASPGLYSERHISGLN
7   ALALEULEULEUARGSERALAGLNPROHIS
8   HISALAGLYGLUVALTHRPHEALACYSARG
9   ASPALAVALALASERALAARGLEUTHRVAL
10   LEUGLYLEUPROASPGLYLEUGLUHISHIS
11   HISHISHISHIS

Samples:

Ig59: Obscurin Ig59, [U-100% 13C; U-100% 15N], 1 mM; D2O, [U-100% 2H], 10%; TRIS 20 mM; sodium chloride 50 mM; sodium azide 350 uM; H2O 90%

sample_conditions_1: ionic strength: 100 mM; pH: 7.5; pressure: 1 atm; temperature: 273 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCIg59isotropicsample_conditions_1
2D 1H-1H TOCSYIg59isotropicsample_conditions_1
3D CBCA(CO)NHIg59isotropicsample_conditions_1
3D C(CO)NHIg59isotropicsample_conditions_1
3D CBCA(CO)NHIg59isotropicsample_conditions_1
3D HNCOIg59isotropicsample_conditions_1
3D HN(CO)CAIg59isotropicsample_conditions_1
3D HNCACBIg59isotropicsample_conditions_1
3D H(CCO)NHIg59isotropicsample_conditions_1

Software:

SPARKY, Goddard - chemical shift assignment, data analysis, peak picking

NMRDraw, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

TOPSPIN v600 MHz, Bruker Biospin - collection

NMR spectrometers:

  • Bruker Avance 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks