BMRB Entry 25930

Title:
Transmembrane domain of human Fas/CD95 death receptor
Deposition date:
2015-12-21
Original release date:
2016-01-25
Authors:
Fu, Qingshan; Chou, James
Citation:

Citation: Fu, Qingshan; Fu, Tianmin; Cruz, Anthony; Sengupta, Prabuddha; Thomas, Stacy; Wang, Shuqing; Siegel, Richard; Wu, Hao; Chou, James. "Structural Basis and Functional Role of Intramembrane Trimerization of the Fas/CD95 Death Receptor"  Mol. Cell 61, 602-613 (2016).
PubMed: 26853147

Assembly members:

Assembly members:
Human_Fas_Transmembrane_Domain, polymer, 28 residues, 3306.097 Da.

Natural source:

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pMM-LR6

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Human_Fas_Transmembrane_Domain: RSNLGWLSLLLLPIPLIVWV KRKEVQKT

Data sets:
Data typeCount
13C chemical shifts51
15N chemical shifts24
1H chemical shifts24

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1entity_11
2entity_21
3entity_31

Entities:

Entity 1, entity_1 28 residues - 3306.097 Da.

1   ARGSERASNLEUGLYTRPLEUSERLEULEU
2   LEULEUPROILEPROLEUILEVALTRPVAL
3   LYSARGLYSGLUVALGLNLYSTHR

Samples:

NC-labeled: Human Fas Transmembrane Domain, [U-100% 13C; U-100% 15N], 1 ± 0.05 mM; DMPC, [U-100% 2H] acyl chains, 60 ± 1 mM; DHPC, [U-100% 2H] acyl chains, 120 ± 5 mM; sodium phosphate 20 ± 1 mM; H2O 95%; D2O 5%

NCD-labeled: Human Fas Transmembrane Domain, [U-100% 13C; U-100% 15N; U-85% 2H], 1 ± 0.05 mM; DMPC 60 ± 1 mM; DHPC 120 ± 5 mM; sodium phosphate 20 ± 1 mM; H2O 95%; D2O 5%

Mix-labeled: Human Fas Transmembrane Domain, [U-100% 15N; U-100% 2H], 0.5 ± 0.05 mM; Human Fas Transmembrane Domain, [U-15% 13C], 0.5 ± 0.05 mM; DMPC, [U-100% 2H] acyl chains, 60 ± 1 mM; DHPC, [U-100% 2H] acyl chains, 120 ± 5 mM; sodium phosphate 20 ± 1 mM; H2O 95%; D2O 5%

sample_conditions_1: ionic strength: 50 mM; pH: 6.8; pressure: 1 atm; temperature: 273 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N TROSY HSQCNCD-labeledisotropicsample_conditions_1
3D TROSY HNCANCD-labeledisotropicsample_conditions_1
3D TROSY HN(CA)CONCD-labeledisotropicsample_conditions_1
3D TROSY HNCONCD-labeledisotropicsample_conditions_1
3D 15N NOESY TROSYNC-labeledisotropicsample_conditions_1
3D 13C NOESY for methylsNC-labeledisotropicsample_conditions_1
3D 15N NOESY TROSYMix-labeledisotropicsample_conditions_1
2D 1H-13C HSQCMix-labeledisotropicsample_conditions_1
2D 1H-15N TROSY HSQCMix-labeledisotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - data analysis, processing

XEASY, Bartels et al. - chemical shift assignment, data analysis

X-PLOR_NIH, Schwieters, Kuszewski, Tjandra and Clore - refinement, structure solution

NMR spectrometers:

  • Bruker Avance 600 MHz
  • Bruker Avance 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks