BMRB Entry 25871

Title:
Apo form of Calmodulin-Like Domain of Human Non-Muscle alpha-actinin 1
Deposition date:
2016-06-27
Original release date:
2016-06-27
Authors:
Drmota Prebil, Sara; Slapsak, Urska; de Almeida Ribeiro, Euripedes; Pavsic, Miha; Ilc, Gregor; Zielinska, Karolina; Hartl, Markus; Backman, Lars; Plavec, Janez; Lenarcic, Brigita; Djinovic-Carugo, Kristina
Citation:

Citation: Drmota Prebil, Sara; Slapsak, Urska; de Almeida Ribeiro, Euripedes; Pavsic, Miha; Ilc, Gregor; Zielinska, Karolina; Hartl, Markus; Backman, Lars; Plavec, Janez; Lenarcic, Brigita; Djinovic-Carugo, Kristina. "Structure and calcium-binding studies of calmodulin-like domain of human non-muscle alpha-actinin-1"  Sci. Rep. 6, 27383-27383 (2016).
PubMed: 27272015

Assembly members:

Assembly members:
Calmodulin-Like_Domain_of_alpha-actinin_1, polymer, 153 residues, 16986.877 Da.

Natural source:

Natural source:   Common Name: humans   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-3d

Data sets:
Data typeCount
13C chemical shifts504
15N chemical shifts152
1H chemical shifts1004

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Calmodulin-Like Domain of alpha-actinin 11

Entities:

Entity 1, Calmodulin-Like Domain of alpha-actinin 1 153 residues - 16986.877 Da.

Residues 1-3 represent a non-native affinity tag This is the GSS linker.

1   GLYSERSERILESERGLNGLUGLNMETASN
2   GLUPHEARGALASERPHEASNHISPHEASP
3   ARGASPHISSERGLYTHRLEUGLYPROGLU
4   GLUPHELYSALACYSLEUILESERLEUGLY
5   TYRASPILEGLYASNASPPROGLNGLYGLU
6   ALAGLUPHEALAARGILEMETSERILEVAL
7   ASPPROASNARGLEUGLYVALVALTHRPHE
8   GLNALAPHEILEASPPHEMETSERARGGLU
9   THRALAASPTHRASPTHRALAASPGLNVAL
10   METALASERPHELYSILELEUALAGLYASP
11   LYSASNTYRILETHRMETASPGLULEUARG
12   ARGGLULEUPROPROASPGLNALAGLUTYR
13   CYSILEALAARGMETALAPROTYRTHRGLY
14   PROASPSERVALPROGLYALALEUASPTYR
15   METSERPHESERTHRALALEUTYRGLYGLU
16   SERASPLEU

Samples:

sample_1: Calmodulin-Like Domain of alpha-actinin 1, [U-100% 13C; U-100% 15N], 1 mM; HEPES 20 mM; Sodium chloride 100 mM; DTT 1.5 mM; EGTA 0.05 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 0.1 M; pH: 7.6; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQC aliphaticsample_1isotropicsample_conditions_1
2D 1H-13C HSQC aromaticsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D C(CO)NHsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
2D 1H-13C HSQC aliphaticsample_1isotropicsample_conditions_1
2D 1H-13C HSQC aromaticsample_1isotropicsample_conditions_1

Software:

CYANA v3.0, Guntert, Mumenthaler and Wuthrich - structure solution

SPARKY v3.113, Goddard - peak picking

TALOS v+, Cornilescu, Delaglio and Bax - Prediction of Protein Backbone Torsion Angles

YASARA, Krieger, Koraimann, Vriend - refinement

NMR spectrometers:

  • Varian VNMRS 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks