BMRB Entry 25380

Title:
Solution structure of HP0268 from Helicobacter pylori
Deposition date:
2014-12-05
Original release date:
2015-12-07
Authors:
Lee, Ki-Young
Citation:

Citation: Lee, Ki-Young; Lee, Kyu-Yeon; Kim, Ji-Hun; Lee, In-Gyun; Lee, Sung-Hee; Sim, Dae-Won; Won, Hyung-Sik; Lee, Bong-Jin. "Structure-based functional identification of Helicobacter pylori HP0268 as a nuclease with both DNA nicking and RNase activities"  Nucleic Acids Res. 43, 5194-5207 (2015).
PubMed: 25916841

Assembly members:

Assembly members:
HP0268, polymer, 82 residues, 9700.074 Da.

Natural source:

Natural source:   Common Name: e-proteobacteria   Taxonomy ID: 210   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Helicobacter pylori

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Helicobacter pylori   Vector: pET28a

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts328
15N chemical shifts80
1H chemical shifts459

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1entity1

Entities:

Entity 1, entity 82 residues - 9700.074 Da.

1   SERHISMETLYSLEUVALLEUALALYSASN
2   THRARGLYSSERASPALALYSSERVALGLU
3   LEUGLUASPLEUTYRHISGLUPHESERGLU
4   ASPLYSARGSERILEPHETYRPHEALAPRO
5   THRASNALAHISLYSASPMETLEULYSALA
6   VALASPPHEPHELYSGLULYSGLYHISTHR
7   ALATYRLEUASPGLUVALARGVALSERTHR
8   ASPGLULYSASPPHELEUTYRGLULEUHIS
9   ILEILE

Samples:

sample_1: sodium phosphate, [U-99% 13C; U-99% 15N], 0.8-1 mM; sodium chloride 50 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 50 mM; pH: 6.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D C(CO)NHsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1

Software:

CYANA, Guntert, Mumenthaler and Wuthrich - structure solution

NMR spectrometers:

  • Bruker DRX 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks