BMRB Entry 25249

Title:
1H, 13C, and 15N Chemical Shift Assignments for the W972A mutant of Arkadia (RNF111) E3 RING domain
Deposition date:
2014-09-25
Original release date:
2017-06-21
Authors:
Chasapis, Christos; Birkou, Maria; Herrmann, Torsten; Episkopou, Vaso; Bentrop, Detlef; Spyroulias, Georgios
Citation:

Citation: Birkou, Maria; Chasapis, Christos; Herrmann, Torsten; Episkopou, Vaso; Bentrop, Detlef; Spyroulias, Georgios. "A residue-specific insight into the Arkadia E3 ubiquitin ligase activity and conformational plasticity"  .

Assembly members:

Assembly members:
W972A, polymer, 69 residues, 7710 Da.
ZINC ION, non-polymer, 65.409 Da.

Natural source:

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pGEX

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts165
15N chemical shifts63
1H chemical shifts193

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1W972A1
2ZINC ION2

Entities:

Entity 1, W972A 69 residues - 7710 Da.

1   METLYSGLNASPGLYGLUGLUGLYTHRGLU
2   GLUASPTHRGLUGLULYSCYSTHRILECYS
3   LEUSERILELEUGLUGLUGLYGLUASPVAL
4   ARGARGLEUPROCYSMETHISLEUPHEHIS
5   GLNVALCYSVALASPGLNALALEUILETHR
6   ASNLYSLYSCYSPROILECYSARGVALASP
7   ILEGLUALAGLNLEUPROSERGLUSER

Entity 2, ZINC ION - Zn - 65.409 Da.

1   ZN

Samples:

sample_1: W972A, [U-99% 15N], 0.6 mM; phosphate buffer 50 mM; H20 90%; D20 10%

sample_2: W972A, [U-98% 13C; U-98% 15N], 0.6 mM; phosphate buffer 50 mM; H20 90%; D20 10%

sample_conditions_1: ionic strength: 50 mM; pH: 7; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCACBsample_2isotropicsample_conditions_1
3D CBCA(CO)NHsample_2isotropicsample_conditions_1
3D HNCAsample_2isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESY aliphaticsample_2isotropicsample_conditions_1
3D 1H-13C NOESY aromaticsample_2isotropicsample_conditions_1
3D HCCH-TOCSYsample_2isotropicsample_conditions_1
APSY 4D-HACANHsample_2isotropicsample_conditions_1
APSY 5D-HACACONHsample_2isotropicsample_conditions_1
APSY 5D-CBCACONHsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_2isotropicsample_conditions_1
3D 1H-13C NOESY aliphaticsample_2isotropicsample_conditions_1
3D 1H-13C NOESY aromaticsample_2isotropicsample_conditions_1

Software:

CARA v1.8.4, Keller and Wuthrich - chemical shift assignment

UNIO v10, Torsten Herrmann - chemical shift assignment

TOPSPIN, Bruker Biospin - collection

NMR spectrometers:

  • Bruker Avance 600 MHz
  • Bruker SB Avance III 1000 MHz

Related Database Links:

UNP Q6ZNA4
AlphaFold Q9NSR1